2jd5

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[[Image:2jd5.png|left|200px]]
 
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{{STRUCTURE_2jd5| PDB=2jd5 | SCENE= }}
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==Sky1p bound to Npl3p-derived substrate peptide==
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<StructureSection load='2jd5' size='340' side='right'caption='[[2jd5]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2jd5]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JD5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2JD5 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2jd5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jd5 OCA], [https://pdbe.org/2jd5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2jd5 RCSB], [https://www.ebi.ac.uk/pdbsum/2jd5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2jd5 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/SKY1_YEAST SKY1_YEAST] Constitutively active kinase, specifically and sequentially phosphorylates serine/arginine (SR)-type shuttling mRNA binding proteins in their RS dipeptide repeats.<ref>PMID:11175909</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jd/2jd5_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2jd5 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The SR protein kinase in yeast, Sky1p, phosphorylates yeast SR-like protein, Npl3p, at a single serine residue located at its C terminus. We report here the X-ray crystal structure of Sky1p bound to a substrate peptide and ADP. Surprisingly, an Npl3p-derived substrate peptide occupies a groove 20 A away from the kinase active site. In vitro studies support the substrate-docking role of this groove. Mutagenesis and binding studies reveal that multiple degenerate short peptide motifs located within the RGG domain of Npl3p serve as the substrate docking motifs. However, a single docking motif is sufficient for its stable interaction with the kinase. Methylation of the docking motifs abolishes kinase binding and phosphorylation of Npl3p. Remarkably, removal of the docking groove in the kinase or the docking motifs of the substrate does not reduce the overall catalytic efficiency of the phosphorylation reaction in any significant manner. We suggest that docking interaction between Sky1p and Npl3p is essential for substrate recruitment and binding specificity.
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===SKY1P BOUND TO NPL3P-DERIVED SUBSTRATE PEPTIDE===
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The RGG domain of Npl3p recruits Sky1p through docking interactions.,Lukasiewicz R, Nolen B, Adams JA, Ghosh G J Mol Biol. 2007 Mar 16;367(1):249-61. Epub 2006 Dec 19. PMID:17239901<ref>PMID:17239901</ref>
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{{ABSTRACT_PUBMED_17239901}}
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 2jd5" style="background-color:#fffaf0;"></div>
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==About this Structure==
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==See Also==
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[[2jd5]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JD5 OCA].
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*[[Serine/threonine protein kinase 3D structures|Serine/threonine protein kinase 3D structures]]
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:017239901</ref><references group="xtra"/>
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__TOC__
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[[Category: Non-specific serine/threonine protein kinase]]
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
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[[Category: Adams, J A.]]
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[[Category: Adams JA]]
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[[Category: Ghosh, G.]]
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[[Category: Ghosh G]]
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[[Category: Huang, D.]]
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[[Category: Huang D]]
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[[Category: Lukasiewicz, R.]]
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[[Category: Lukasiewicz R]]
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[[Category: Nolen, B.]]
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[[Category: Nolen B]]
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[[Category: Atp-binding]]
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[[Category: Kinase]]
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[[Category: Mrna export protein]]
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[[Category: Nucleotide-binding]]
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[[Category: Serine/threonine-protein kinase]]
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[[Category: Transferase]]
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Current revision

Sky1p bound to Npl3p-derived substrate peptide

PDB ID 2jd5

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