2f7t
From Proteopedia
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| - | [[Image:2f7t.png|left|200px]] | ||
| - | + | ==Crystal structure of the catalytic domain of Mos1 mariner transposase== | |
| + | <StructureSection load='2f7t' size='340' side='right'caption='[[2f7t]], [[Resolution|resolution]] 2.25Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[2f7t]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_mauritiana Drosophila mauritiana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2F7T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2F7T FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.25Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2f7t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2f7t OCA], [https://pdbe.org/2f7t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2f7t RCSB], [https://www.ebi.ac.uk/pdbsum/2f7t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2f7t ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/MOS1T_DROMA MOS1T_DROMA] Mediates transposition of transposon Mos1 by a 'cut and paste' mechanism. Transposases are sequence-specific nucleases and strand transferases that catalyze transposition through an ordered series of events: sequence-specific binding of transposase to the terminal inverted repeats (IR) present at each end of the transposon, pairing of the transposon IRs in a paired-end complex (PEC), cleavage of one or both DNA strands at each transposon end, capture of target DNA, and strand transfer to insert the transposon at a new site. | ||
| + | == Evolutionary Conservation == | ||
| + | [[Image:Consurf_key_small.gif|200px|right]] | ||
| + | Check<jmol> | ||
| + | <jmolCheckbox> | ||
| + | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/f7/2f7t_consurf.spt"</scriptWhenChecked> | ||
| + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | ||
| + | <text>to colour the structure by Evolutionary Conservation</text> | ||
| + | </jmolCheckbox> | ||
| + | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2f7t ConSurf]. | ||
| + | <div style="clear:both"></div> | ||
| - | == | + | ==See Also== |
| - | + | *[[Transposase 3D structures|Transposase 3D structures]] | |
| - | + | __TOC__ | |
| - | + | </StructureSection> | |
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| - | [[ | + | |
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| - | < | + | |
[[Category: Drosophila mauritiana]] | [[Category: Drosophila mauritiana]] | ||
| - | [[Category: Dawson | + | [[Category: Large Structures]] |
| - | [[Category: Finnegan | + | [[Category: Dawson A]] |
| - | [[Category: Richardson | + | [[Category: Finnegan DJ]] |
| - | [[Category: Taylor | + | [[Category: Richardson JM]] |
| - | [[Category: Walkinshaw | + | [[Category: Taylor P]] |
| - | + | [[Category: Walkinshaw MD]] | |
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Current revision
Crystal structure of the catalytic domain of Mos1 mariner transposase
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