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2nmb

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[[Image:2nmb.png|left|200px]]
 
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{{STRUCTURE_2nmb| PDB=2nmb | SCENE= }}
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==DNUMB PTB DOMAIN COMPLEXED WITH A PHOSPHOTYROSINE PEPTIDE, NMR, ENSEMBLE OF STRUCTURES.==
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<StructureSection load='2nmb' size='340' side='right'caption='[[2nmb]]' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2nmb]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NMB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2NMB FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PTR:O-PHOSPHOTYROSINE'>PTR</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2nmb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2nmb OCA], [https://pdbe.org/2nmb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2nmb RCSB], [https://www.ebi.ac.uk/pdbsum/2nmb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2nmb ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/NUMB_DROME NUMB_DROME] Required in determination of cell fate during sensory organ formation in embryos. Functions in nuclei and seems to interact with nucleic acids.<ref>PMID:2752427</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/nm/2nmb_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2nmb ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The phosphotyrosine-binding (PTB) domain of Numb, a protein involved in asymmetric cell division, has recently been shown to bind to the adapter protein Lnx through an LDNPAY sequence, to the Numb-associated kinase (Nak) through a sequence that does not contain an NPXY motif and to GP(p)Y-containing peptides obtained from library screening. We show here that these diverse peptide sequences bind with comparable affinities to the Numb PTB domain at a common binding site on the surface of the protein. The NMR structure of the Numb PTB domain in complex with a GPpY-containing peptide reveals a novel mechanism of binding with the peptide in a helical turn that does not hydrogen bond to the PTB domain beta-sheet. These results suggest that PTB domains can potentially have multiple modes of peptide recognition and provide a structural basis from which the multiple functions of the Numb PTB domain during asymmetric cell division could arise.
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===DNUMB PTB DOMAIN COMPLEXED WITH A PHOSPHOTYROSINE PEPTIDE, NMR, ENSEMBLE OF STRUCTURES.===
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Structure of a Numb PTB domain-peptide complex suggests a basis for diverse binding specificity.,Li SC, Zwahlen C, Vincent SJ, McGlade CJ, Kay LE, Pawson T, Forman-Kay JD Nat Struct Biol. 1998 Dec;5(12):1075-83. PMID:9846878<ref>PMID:9846878</ref>
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{{ABSTRACT_PUBMED_9846878}}
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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==About this Structure==
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<div class="pdbe-citations 2nmb" style="background-color:#fffaf0;"></div>
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[[2nmb]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NMB OCA].
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== References ==
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<references/>
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==Reference==
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__TOC__
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<ref group="xtra">PMID:009846878</ref><references group="xtra"/>
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</StructureSection>
[[Category: Drosophila melanogaster]]
[[Category: Drosophila melanogaster]]
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[[Category: Forman-Kay, J D.]]
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[[Category: Large Structures]]
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[[Category: Li, S C.]]
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[[Category: Forman-Kay JD]]
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[[Category: McGlade, C J.]]
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[[Category: Li S-C]]
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[[Category: Pawson,T.]]
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[[Category: McGlade CJ]]
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[[Category: Vincent, S J.F.]]
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[[Category: Pawson T]]
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[[Category: Zwahlen, C.]]
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[[Category: Vincent SJF]]
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[[Category: Asymetr ic cell division]]
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[[Category: Zwahlen C]]
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[[Category: Cell cycle-gene regulation complex]]
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[[Category: Complex]]
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[[Category: Signal transduction]]
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Current revision

DNUMB PTB DOMAIN COMPLEXED WITH A PHOSPHOTYROSINE PEPTIDE, NMR, ENSEMBLE OF STRUCTURES.

PDB ID 2nmb

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