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3cr8

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[[Image:3cr8.png|left|200px]]
 
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{{STRUCTURE_3cr8| PDB=3cr8 | SCENE= }}
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==Hexameric APS kinase from Thiobacillus denitrificans==
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<StructureSection load='3cr8' size='340' side='right'caption='[[3cr8]], [[Resolution|resolution]] 2.95&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3cr8]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Thiobacillus_denitrificans Thiobacillus denitrificans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CR8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3CR8 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.95&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3cr8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3cr8 OCA], [https://pdbe.org/3cr8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3cr8 RCSB], [https://www.ebi.ac.uk/pdbsum/3cr8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3cr8 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q3SM86_THIDA Q3SM86_THIDA]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cr/3cr8_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3cr8 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The Tbd_0210 gene of the chemolithotrophic bacterium Thiobacillus denitrificans is annotated to encode a 60.5 kDa bifunctional enzyme with ATP sulfurylase and APS kinase activity. This putative bifunctional enzyme was cloned, expressed and structurally characterized. The 2.95 A resolution X-ray crystal structure reported here revealed a hexameric assembly with D(3) symmetry. Each subunit contains a large N-terminal sulfurylase-like domain and a C-terminal APS kinase domain reminiscent of the two-domain fungal ATP sulfurylases of Penicillium chrysogenum and Saccharomyces cerevisiae, which also exhibit a hexameric assembly. However, the T. denitrificans enzyme exhibits numerous structural and sequence differences in the N-terminal domain that render it inactive with respect to ATP sulfurylase activity. Surprisingly, the C-terminal domain does indeed display APS kinase activity, indicating that this gene product is a true APS kinase. Therefore, these results provide the first structural insights into a unique hexameric APS kinase that contains a nonfunctional ATP sulfurylase-like domain of unknown function.
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===Hexameric APS kinase from Thiobacillus denitrificans===
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Structure of the two-domain hexameric APS kinase from Thiobacillus denitrificans: structural basis for the absence of ATP sulfurylase activity.,Gay SC, Segel IH, Fisher AJ Acta Crystallogr D Biol Crystallogr. 2009 Oct;65(Pt 10):1021-31. Epub 2009, Sep 16. PMID:19770499<ref>PMID:19770499</ref>
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{{ABSTRACT_PUBMED_19770499}}
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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==About this Structure==
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<div class="pdbe-citations 3cr8" style="background-color:#fffaf0;"></div>
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[[3cr8]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Thiobacillus_denitrificans Thiobacillus denitrificans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CR8 OCA].
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== References ==
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<references/>
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==Reference==
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__TOC__
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<ref group="xtra">PMID:019770499</ref><references group="xtra"/>
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Thiobacillus denitrificans]]
[[Category: Thiobacillus denitrificans]]
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[[Category: Fisher, A J.]]
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[[Category: Fisher AJ]]
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[[Category: Gay, S C.]]
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[[Category: Gay SC]]
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[[Category: Segel, I H.]]
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[[Category: Segel IH]]
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[[Category: Adenylylsulfate kinase]]
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[[Category: Aps kinase]]
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[[Category: Nucleotide 2 kinase]]
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[[Category: Nucleotidyltransferase]]
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[[Category: Sulfate metabolism]]
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[[Category: Transferase]]
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Current revision

Hexameric APS kinase from Thiobacillus denitrificans

PDB ID 3cr8

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