3b2m

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[[Image:3b2m.png|left|200px]]
 
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{{STRUCTURE_3b2m| PDB=3b2m | SCENE= }}
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==Crystal Structure of the Major Pilin from Streptococcus pyogenes==
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<StructureSection load='3b2m' size='340' side='right'caption='[[3b2m]], [[Resolution|resolution]] 2.22&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3b2m]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_pyogenes_serotype_M1 Streptococcus pyogenes serotype M1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3B2M OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3B2M FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.22&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3b2m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3b2m OCA], [https://pdbe.org/3b2m PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3b2m RCSB], [https://www.ebi.ac.uk/pdbsum/3b2m PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3b2m ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/PILIN_STRP1 PILIN_STRP1] Major component of the pilus. A stack of the pilin subunits, joined by intermolecular isopeptide bonds, forms the pilus. The pilus is required for bacterial adhesion to host cells, for bacterial aggregation, and for biofilm formation.<ref>PMID:17501921</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/b2/3b2m_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3b2m ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Many bacterial pathogens have long, slender pili through which they adhere to host cells. The crystal structure of the major pilin subunit from the Gram-positive human pathogen Streptococcus pyogenes at 2.2 angstroms resolution reveals an extended structure comprising two all-beta domains. The molecules associate in columns through the crystal, with each carboxyl terminus adjacent to a conserved lysine of the next molecule. This lysine forms the isopeptide bonds that link the subunits in native pili, validating the relevance of the crystal assembly. Each subunit contains two lysine-asparagine isopeptide bonds generated by an intramolecular reaction, and we find evidence for similar isopeptide bonds in other cell surface proteins of Gram-positive bacteria. The present structure explains the strength and stability of such Gram-positive pili and could facilitate vaccine development.
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===Crystal Structure of the Major Pilin from Streptococcus pyogenes===
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Stabilizing isopeptide bonds revealed in gram-positive bacterial pilus structure.,Kang HJ, Coulibaly F, Clow F, Proft T, Baker EN Science. 2007 Dec 7;318(5856):1625-8. PMID:18063798<ref>PMID:18063798</ref>
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{{ABSTRACT_PUBMED_18063798}}
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3b2m" style="background-color:#fffaf0;"></div>
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==About this Structure==
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==See Also==
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[[3b2m]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptococcus_pyogenes_serotype_m1 Streptococcus pyogenes serotype m1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3B2M OCA].
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*[[Pilin 3D structures|Pilin 3D structures]]
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:018063798</ref><references group="xtra"/>
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__TOC__
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[[Category: Streptococcus pyogenes serotype m1]]
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</StructureSection>
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[[Category: Baker, E N.]]
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[[Category: Large Structures]]
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[[Category: Coulibaly, F.]]
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[[Category: Streptococcus pyogenes serotype M1]]
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[[Category: Kang, H J.]]
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[[Category: Baker EN]]
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[[Category: Proft, T.]]
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[[Category: Coulibaly F]]
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[[Category: All-beta]]
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[[Category: Kang HJ]]
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[[Category: Cell adhesion]]
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[[Category: Proft T]]
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[[Category: Isopeptide]]
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[[Category: Pili]]
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[[Category: Structural protein]]
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Current revision

Crystal Structure of the Major Pilin from Streptococcus pyogenes

PDB ID 3b2m

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