3cfc

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[[Image:3cfc.png|left|200px]]
 
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{{STRUCTURE_3cfc| PDB=3cfc | SCENE= }}
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==High-resolution structure of blue fluorescent antibody EP2-19G2==
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<StructureSection load='3cfc' size='340' side='right'caption='[[3cfc]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3cfc]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CFC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3CFC FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3cfc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3cfc OCA], [https://pdbe.org/3cfc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3cfc RCSB], [https://www.ebi.ac.uk/pdbsum/3cfc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3cfc ProSAT]</span></td></tr>
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</table>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cf/3cfc_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3cfc ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The blue-emissive antibody EP2-19G2 that has been elicited against trans-stilbene has unprecedented ability to produce bright luminescence and has been used as a biosensor in various applications. We show that the prolonged luminescence is not stilbene fluorescence. Instead, the emissive species is a charge-transfer excited complex of an anionic stilbene and a cationic, parallel pi-stacked tryptophan. Upon charge recombination, this complex generates exceptionally bright blue light. Complex formation is enabled by a deeply penetrating ligand-binding pocket, which in turn results from a noncanonical interface between the two variable domains of the antibody.
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===High-resolution structure of blue fluorescent antibody EP2-19G2===
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Deeply inverted electron-hole recombination in a luminescent antibody-stilbene complex.,Debler EW, Kaufmann GF, Meijler MM, Heine A, Mee JM, Pljevaljcic G, Di Bilio AJ, Schultz PG, Millar DP, Janda KD, Wilson IA, Gray HB, Lerner RA Science. 2008 Feb 29;319(5867):1232-5. PMID:18309081<ref>PMID:18309081</ref>
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{{ABSTRACT_PUBMED_18309081}}
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3cfc" style="background-color:#fffaf0;"></div>
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==About this Structure==
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==See Also==
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[[3cfc]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CFC OCA].
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*[[Antibody 3D structures|Antibody 3D structures]]
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*[[Sandbox 20009|Sandbox 20009]]
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==Reference==
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*[[3D structures of non-human antibody|3D structures of non-human antibody]]
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<ref group="xtra">PMID:018309081</ref><references group="xtra"/>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Mus musculus]]
[[Category: Mus musculus]]
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[[Category: Debler, E W.]]
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[[Category: Debler EW]]
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[[Category: Wilson, I A.]]
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[[Category: Wilson IA]]
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[[Category: Blue-fluorescent antibody]]
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[[Category: Electron transfer]]
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[[Category: Hapten complex]]
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[[Category: Immune system]]
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[[Category: Immunoglobulin]]
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Current revision

High-resolution structure of blue fluorescent antibody EP2-19G2

PDB ID 3cfc

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Proteopedia Page Contributors and Editors (what is this?)

OCA

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