2zjb

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[[Image:2zjb.png|left|200px]]
 
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{{STRUCTURE_2zjb| PDB=2zjb | SCENE= }}
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==Crystal structure of the human Dmc1-M200V polymorphic variant==
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<StructureSection load='2zjb' size='340' side='right'caption='[[2zjb]], [[Resolution|resolution]] 3.50&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2zjb]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZJB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2ZJB FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.5&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2zjb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zjb OCA], [https://pdbe.org/2zjb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2zjb RCSB], [https://www.ebi.ac.uk/pdbsum/2zjb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2zjb ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/DMC1_HUMAN DMC1_HUMAN] May participate in meiotic recombination, specifically in homologous strand assimilation, which is required for the resolution of meiotic double-strand breaks (By similarity).
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/zj/2zjb_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2zjb ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The M200V polymorphism of the human DMC1 protein, which is an essential, meiosis-specific DNA recombinase, was found in an infertile patient, raising the question of whether this homozygous human DMC1-M200V polymorphism may cause infertility by affecting the function of the human DMC1 protein. In the present study, we determined the crystal structure of the human DMC1-M200V variant in the octameric-ring form. Biochemical analyses revealed that the human DMC1-M200V variant had reduced stability, and was moderately defective in catalyzing in vitro recombination reactions. The corresponding M194V mutation introduced in the Schizosaccharomyces pombe dmc1 gene caused a significant decrease in the meiotic homologous recombination frequency. Together, these structural, biochemical and genetic results provide extensive evidence that the human DMC1-M200V mutation impairs its function, supporting the previous interpretation that this single-nucleotide polymorphism is a source of human infertility.
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===Crystal structure of the human Dmc1-M200V polymorphic variant===
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Structural and functional analyses of the DMC1-M200V polymorphism found in the human population.,Hikiba J, Hirota K, Kagawa W, Ikawa S, Kinebuchi T, Sakane I, Takizawa Y, Yokoyama S, Mandon-Pepin B, Nicolas A, Shibata T, Ohta K, Kurumizaka H Nucleic Acids Res. 2008 Jul;36(12):4181-90. Epub 2008 Jun 19. PMID:18566005<ref>PMID:18566005</ref>
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{{ABSTRACT_PUBMED_18566005}}
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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==About this Structure==
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<div class="pdbe-citations 2zjb" style="background-color:#fffaf0;"></div>
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[[2zjb]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZJB OCA].
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== References ==
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<references/>
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==Reference==
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__TOC__
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<ref group="xtra">PMID:018566005</ref><references group="xtra"/>
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Hikiba, J.]]
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[[Category: Large Structures]]
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[[Category: Hirota, K.]]
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[[Category: Hikiba J]]
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[[Category: Ikawa, S.]]
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[[Category: Hirota K]]
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[[Category: Kagawa, W.]]
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[[Category: Ikawa S]]
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[[Category: Kinebuchi, T.]]
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[[Category: Kagawa W]]
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[[Category: Kurumizaka, H.]]
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[[Category: Kinebuchi T]]
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[[Category: Mandon-Pepin, B.]]
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[[Category: Kurumizaka H]]
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[[Category: Nicolas, A.]]
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[[Category: Mandon-Pepin B]]
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[[Category: Ohta, K.]]
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[[Category: Nicolas A]]
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[[Category: Sakane, I.]]
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[[Category: Ohta K]]
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[[Category: Shibata, T.]]
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[[Category: Sakane I]]
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[[Category: Takizawa, Y.]]
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[[Category: Shibata T]]
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[[Category: Yokoyama, S.]]
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[[Category: Takizawa Y]]
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[[Category: Aaa atpase]]
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[[Category: Yokoyama S]]
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[[Category: Atp-binding]]
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[[Category: Cell cycle]]
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[[Category: Dna-binding protein]]
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[[Category: Meiosis]]
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[[Category: Nucleotide-binding]]
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[[Category: Nucleus]]
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[[Category: Octamer]]
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[[Category: Recombination]]
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[[Category: Ring protein]]
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Current revision

Crystal structure of the human Dmc1-M200V polymorphic variant

PDB ID 2zjb

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