2x7z

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[[Image:2x7z.png|left|200px]]
 
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{{STRUCTURE_2x7z| PDB=2x7z | SCENE= }}
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==Crystal Structure of the SAP97 PDZ2 I342W C378A mutant protein domain==
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<StructureSection load='2x7z' size='340' side='right'caption='[[2x7z]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2x7z]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2X7Z OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2X7Z FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=IMD:IMIDAZOLE'>IMD</scene>, <scene name='pdbligand=NH4:AMMONIUM+ION'>NH4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2x7z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2x7z OCA], [https://pdbe.org/2x7z PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2x7z RCSB], [https://www.ebi.ac.uk/pdbsum/2x7z PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2x7z ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/DLG1_HUMAN DLG1_HUMAN] Essential multidomain scaffolding protein required for normal development (By similarity). Recruits channels, receptors and signaling molecules to discrete plasma membrane domains in polarized cells. May play a role in adherens junction assembly, signal transduction, cell proliferation, synaptogenesis and lymphocyte activation. Regulates the excitability of cardiac myocytes by modulating the functional expression of Kv4 channels. Functional regulator of Kv1.5 channel.<ref>PMID:10656683</ref> <ref>PMID:12445884</ref> <ref>PMID:14699157</ref> <ref>PMID:15263016</ref> <ref>PMID:19213956</ref> <ref>PMID:20605917</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/x7/2x7z_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2x7z ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Protein domains usually fold without or with only transiently populated intermediates, possibly to avoid misfolding, which could result in amyloidogenic disease. Whether observed intermediates are productive and obligatory species on the folding reaction pathway or dispensable by-products is a matter of debate. Here, we solved the crystal structure of a small protein domain, SAP97 PDZ2 I342W C378A, and determined its folding pathway. The presence of a folding intermediate was demonstrated both by single and double-mixing kinetic experiments using urea-induced (un)folding as well as ligand-induced folding. This protein domain was found to fold via a triangular scheme, where the folding intermediate could be either on- or off-pathway, depending on the experimental conditions. Furthermore, we found that the intermediate was present at equilibrium, which is rarely seen in folding reactions of small protein domains. The folding mechanism observed here illustrates the roughness and plasticity of the protein folding energy landscape, where several routes may be employed to reach the native state. The results also reconcile the folding mechanisms of topological variants within the PDZ domain family.
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===CRYSTAL STRUCTURE OF THE SAP97 PDZ2 I342W C378A MUTANT PROTEIN DOMAIN===
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The plastic energy landscape of protein folding: a triangular folding mechanism with an equilibrium intermediate for a small protein domain.,Haq SR, Jurgens MC, Chi CN, Koh CS, Elfstrom L, Selmer M, Gianni S, Jemth P J Biol Chem. 2010 Jun 4;285(23):18051-9. Epub 2010 Mar 30. PMID:20356847<ref>PMID:20356847</ref>
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{{ABSTRACT_PUBMED_20356847}}
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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==About this Structure==
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<div class="pdbe-citations 2x7z" style="background-color:#fffaf0;"></div>
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[[2x7z]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2X7Z OCA].
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== References ==
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<references/>
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==Reference==
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__TOC__
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<ref group="xtra">PMID:020356847</ref><references group="xtra"/>
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Chi, C N.]]
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[[Category: Large Structures]]
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[[Category: Elfstrom, L.]]
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[[Category: Chi CN]]
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[[Category: Gianni, S.]]
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[[Category: Elfstrom L]]
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[[Category: Haq, S R.]]
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[[Category: Gianni S]]
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[[Category: Jemth, P.]]
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[[Category: Haq SR]]
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[[Category: Jurgens, M C.]]
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[[Category: Jemth P]]
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[[Category: Koh, C S.]]
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[[Category: Jurgens MC]]
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[[Category: Selmer, M.]]
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[[Category: Koh CS]]
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[[Category: Host-virus interaction]]
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[[Category: Selmer M]]
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[[Category: Phosphoprotein]]
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[[Category: Sh3 domain]]
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[[Category: Structural protein]]
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[[Category: Synaptic protein]]
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Current revision

Crystal Structure of the SAP97 PDZ2 I342W C378A mutant protein domain

PDB ID 2x7z

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