2wzl

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
m (Protected "2wzl" [edit=sysop:move=sysop])
Current revision (10:20, 20 December 2023) (edit) (undo)
 
(6 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:2wzl.png|left|200px]]
 
-
{{STRUCTURE_2wzl| PDB=2wzl | SCENE= }}
+
==The Structure of the N-RNA Binding Domain of the Mokola virus Phosphoprotein==
 +
<StructureSection load='2wzl' size='340' side='right'caption='[[2wzl]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[2wzl]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mokola_lyssavirus Mokola lyssavirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WZL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2WZL FirstGlance]. <br>
 +
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
 +
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2wzl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wzl OCA], [https://pdbe.org/2wzl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2wzl RCSB], [https://www.ebi.ac.uk/pdbsum/2wzl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2wzl ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/PHOSP_MOKV PHOSP_MOKV] Non catalytic polymerase cofactor and regulatory protein that plays a role in viral transcription and replication. Stabilizes the RNA polymerase L to the N-RNA template and binds the soluble protein N, preventing it from encapsidating non-genomic RNA. Also inhibits host IFN-alpha and IFN-beta signaling by binding and retaining phosphorylated STAT1 in the cytoplasm or by inhibiting the DNA binding of STAT1 in the nucleus (By similarity).
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/wz/2wzl_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2wzl ConSurf].
 +
<div style="clear:both"></div>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
Mokola virus (MOKV) is a nonsegmented, negative-sense RNA virus that belongs to the Lyssavirus genus and Rhabdoviridae family. MOKV phosphoprotein P is an essential component of the replication and transcription complex and acts as a cofactor for the viral RNA-dependent RNA polymerase. P recruits the viral polymerase to the nucleoprotein-bound viral RNA (N-RNA) via an interaction between its C-terminal domain and the N-RNA complex. Here we present a structure for this domain of MOKV P, obtained by expression of full-length P in Escherichia coli, which was subsequently truncated during crystallization. The structure has a high degree of homology with P of rabies virus, another member of Lyssavirus genus, and to a lesser degree with P of vesicular stomatitis virus (VSV), a member of the related Vesiculovirus genus. In addition, analysis of the crystal packing of this domain reveals a potential binding site for the nucleoprotein N. Using both site-directed mutagenesis and yeast two-hybrid experiments to measure P-N interaction, we have determined the relative roles of key amino acids involved in this interaction to map the region of P that binds N. This analysis also reveals a structural relationship between the N-RNA binding domain of the P proteins of the Rhabdoviridae and the Paramyxoviridae.
-
===THE STRUCTURE OF THE N-RNA BINDING DOMAIN OF THE MOKOLA VIRUS PHOSPHOPROTEIN===
+
Structure of the nucleoprotein binding domain of Mokola virus phosphoprotein.,Assenberg R, Delmas O, Ren J, Vidalain PO, Verma A, Larrous F, Graham SC, Tangy F, Grimes JM, Bourhy H J Virol. 2010 Jan;84(2):1089-96. Epub 2009 Nov 11. PMID:19906936<ref>PMID:19906936</ref>
-
{{ABSTRACT_PUBMED_19906936}}
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
 
+
</div>
-
==About this Structure==
+
<div class="pdbe-citations 2wzl" style="background-color:#fffaf0;"></div>
-
[[2wzl]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Mokola_virus Mokola virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WZL OCA].
+
== References ==
-
 
+
<references/>
-
==Reference==
+
__TOC__
-
<ref group="xtra">PMID:019906936</ref><references group="xtra"/>
+
</StructureSection>
-
[[Category: Mokola virus]]
+
[[Category: Large Structures]]
-
[[Category: Assenberg, R.]]
+
[[Category: Mokola lyssavirus]]
-
[[Category: Bourhy, H.]]
+
[[Category: Assenberg R]]
-
[[Category: Delmas, O.]]
+
[[Category: Bourhy H]]
-
[[Category: Graham, S.]]
+
[[Category: Delmas O]]
-
[[Category: Grimes, J.]]
+
[[Category: Graham S]]
-
[[Category: Larrous, F.]]
+
[[Category: Grimes J]]
-
[[Category: Ren, J.]]
+
[[Category: Larrous F]]
-
[[Category: Tangy, F.]]
+
[[Category: Ren J]]
-
[[Category: Verma, A.]]
+
[[Category: Tangy F]]
-
[[Category: Vidalain, P.]]
+
[[Category: Verma A]]
-
[[Category: Chaperone]]
+
[[Category: Vidalain P]]
-
[[Category: Nucleoprotein]]
+
-
[[Category: Rabies]]
+
-
[[Category: Rna replication]]
+
-
[[Category: Viral protein]]
+
-
[[Category: Virion]]
+

Current revision

The Structure of the N-RNA Binding Domain of the Mokola virus Phosphoprotein

PDB ID 2wzl

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2wzl"
Personal tools