2vdw

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[[Image:2vdw.png|left|200px]]
 
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{{STRUCTURE_2vdw| PDB=2vdw | SCENE= }}
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==Guanosine N7 methyl-transferase sub-complex (D1-D12) of the vaccinia virus mRNA capping enzyme==
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<StructureSection load='2vdw' size='340' side='right'caption='[[2vdw]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2vdw]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Vaccinia_virus Vaccinia virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VDW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2VDW FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2vdw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vdw OCA], [https://pdbe.org/2vdw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2vdw RCSB], [https://www.ebi.ac.uk/pdbsum/2vdw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2vdw ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/MCEL_VACCW MCEL_VACCW] Catalytic subunit of the mRNA capping enzyme which catalyzes three enzymatic reactions: the 5' triphosphate end of the pre-mRNA is hydrolyzed to a diphosphate by RNA 5' triphosphatase; the diphosphate RNA end is capped with GMP by RNA guanylyltransferase and the GpppN cap is methylated by RNA (guanine-N7) methyltransferase. Heterodimeric mRNA capping enzyme catalyzes the linkage of a N7-methyl-guanosine moiety to the first transcribed nucleotide (cap 0 structure), whereas the polymerase associated VP39 is responsible for a second methylation at the 2'-O position of the ribose (cap 1 structure).<ref>PMID:18295814</ref> <ref>PMID:18455214</ref> <ref>PMID:18256245</ref> The heterodimeric enzyme is also involved in early viral gene transcription termination and intermediate viral gene transcription initiation. Early gene transcription termination requires the termination factor VTF, the DNA-dependent ATPase NPH-I and the Rap94 subunit of the viral RNA polymerase, as well as the presence of a specific termination motif. Binds, together with RAP94, to the termination motif 5'-UUUUUNU-3' in the nascent early mRNA.<ref>PMID:18295814</ref> <ref>PMID:18455214</ref> <ref>PMID:18256245</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/vd/2vdw_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2vdw ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The vaccinia virus mRNA capping enzyme is a multifunctional heterodimeric protein associated with the viral polymerase that both catalyses the three steps of mRNA capping and regulates gene transcription. The structure of a subcomplex comprising the C-terminal N7-methyl-transferase (MT) domain of the large D1 subunit, the stimulatory D12 subunit and bound S-adenosyl-homocysteine (AdoHcy) has been determined at 2.7 A resolution and reveals several novel features of the poxvirus capping enzyme. The structure shows for the first time the critical role played by the proteolytically sensitive N-terminus of the MT domain in binding the methyl donor and in catalysis. In addition, the poxvirus enzyme has a completely unique mode of binding of the adenosine moiety of AdoHcy, a feature that could be exploited for design of specific anti-poxviral compounds. The structure of the poxvirus-specific D12 subunit suggests that it was originally an RNA cap 2'O-MT that has evolved to a catalytically inactive form that has been retained for D1 stabilisation and MT activity enhancement through an allosteric mechanism.
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===GUANOSINE N7 METHYL-TRANSFERASE SUB-COMPLEX (D1-D12) OF THE VACCINIA VIRUS MRNA CAPPING ENZYME===
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Structural insights into the mechanism and evolution of the vaccinia virus mRNA cap N7 methyl-transferase.,De la Pena M, Kyrieleis OJ, Cusack S EMBO J. 2007 Nov 28;26(23):4913-25. Epub 2007 Nov 8. PMID:17989694<ref>PMID:17989694</ref>
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{{ABSTRACT_PUBMED_17989694}}
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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==About this Structure==
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<div class="pdbe-citations 2vdw" style="background-color:#fffaf0;"></div>
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[[2vdw]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Vaccinia_virus Vaccinia virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VDW OCA].
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== References ==
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<references/>
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==Reference==
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__TOC__
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<ref group="xtra">PMID:017989694</ref><references group="xtra"/>
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Vaccinia virus]]
[[Category: Vaccinia virus]]
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[[Category: MRNA guanylyltransferase]]
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[[Category: Cusack S]]
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[[Category: Cusack, S.]]
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[[Category: De la Pena M]]
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[[Category: Kyrieleis, O J.P.]]
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[[Category: Kyrieleis OJP]]
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[[Category: Pena, M De La.]]
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[[Category: D1-d12 heterodimer]]
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[[Category: Hydrolase]]
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[[Category: Methyl-transferase]]
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[[Category: Methyltransferase]]
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[[Category: Mrna capping]]
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[[Category: Mrna capping enzyme]]
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[[Category: Mrna processing]]
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[[Category: Multifunctional enzyme]]
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[[Category: Nucleotidyltransferase]]
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[[Category: Poxvirus]]
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[[Category: Rna metabolism]]
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[[Category: S-adenosyl homocysteine]]
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[[Category: S-adenosyl-l-methionine]]
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[[Category: Transferase]]
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Current revision

Guanosine N7 methyl-transferase sub-complex (D1-D12) of the vaccinia virus mRNA capping enzyme

PDB ID 2vdw

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