2w48

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[[Image:2w48.png|left|200px]]
 
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{{STRUCTURE_2w48| PDB=2w48 | SCENE= }}
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==Crystal structure of the Full-length Sorbitol Operon Regulator SorC from Klebsiella pneumoniae==
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<StructureSection load='2w48' size='340' side='right'caption='[[2w48]], [[Resolution|resolution]] 3.20&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2w48]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Klebsiella_pneumoniae Klebsiella pneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2W48 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2W48 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=MRD:(4R)-2-METHYLPENTANE-2,4-DIOL'>MRD</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2w48 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2w48 OCA], [https://pdbe.org/2w48 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2w48 RCSB], [https://www.ebi.ac.uk/pdbsum/2w48 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2w48 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/SORC_KLEPN SORC_KLEPN] Positively regulates, in the presence of L-sorbose, and negatively regulates, in the absence of L-sorbose, the transcription of the sor operon.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/w4/2w48_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2w48 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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SorC transcriptional regulators are common regulators in prokaryotes. Here we report the first crystal structure of a full-length SorC, the sorbitol operon regulator SorC from Klebsiella pneumoniae, the prototype of its family. SorC was found to be a homotetramer (which seems to be the biologically active form) that is able to recognize its DNA operator. The tetramer can be regarded as a dimer of dimers, with each dimer being composed of two identical subunits in different conformations. The DNA-binding domains divergently protrude from the core of the tetramer, suggesting that SorC may bind its operator in two distinct regions. The sugar-binding domain presents the same fold identified in members of the SorC family that shows some features identified as specific for sugar recognition. An in silico analysis shows that the localization of the putative sugar-binding site is close to the dimeric interfaces. This supports the proposal of a new mechanism of transcriptional regulation that is in complete agreement with functional studies recently reported on a protein belonging to the same family.
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===CRYSTAL STRUCTURE OF THE FULL-LENGTH SORBITOL OPERON REGULATOR SORC FROM KLEBSIELLA PNEUMONIAE===
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Crystal structure of the full-length sorbitol operon regulator SorC from Klebsiella pneumoniae: structural evidence for a novel transcriptional regulation mechanism.,de Sanctis D, McVey CE, Enguita FJ, Carrondo MA J Mol Biol. 2009 Apr 3;387(3):759-70. Epub 2009 Feb 14. PMID:19232357<ref>PMID:19232357</ref>
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{{ABSTRACT_PUBMED_19232357}}
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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==About this Structure==
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<div class="pdbe-citations 2w48" style="background-color:#fffaf0;"></div>
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[[2w48]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Klebsiella_pneumoniae Klebsiella pneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2W48 OCA].
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== References ==
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<references/>
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==Reference==
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__TOC__
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<ref group="xtra">PMID:019232357</ref><references group="xtra"/>
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</StructureSection>
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[[Category: Klebsiella pneumoniae]]
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[[Category: Carrondo, M A.]]
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[[Category: Enguita, F J.]]
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[[Category: Mcvey, C E.]]
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[[Category: Sanctis, D De.]]
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[[Category: Activator]]
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[[Category: Dna-binding]]
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[[Category: Klebsiella pneumoniae]]
[[Category: Klebsiella pneumoniae]]
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[[Category: Repressor]]
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[[Category: Large Structures]]
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[[Category: Sorc]]
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[[Category: Carrondo MA]]
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[[Category: Transcription]]
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[[Category: Enguita FJ]]
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[[Category: Transcription regulation]]
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[[Category: McVey CE]]
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[[Category: Transcription regulator]]
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[[Category: De Sanctis D]]

Current revision

Crystal structure of the Full-length Sorbitol Operon Regulator SorC from Klebsiella pneumoniae

PDB ID 2w48

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