1xam

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (08:48, 14 February 2024) (edit) (undo)
 
(14 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1xam.gif|left|200px]]<br /><applet load="1xam" size="350" color="white" frame="true" align="right" spinBox="true"
 
-
caption="1xam, resolution 1.86&Aring;" />
 
-
'''Cobalt hexammine induced tautameric shift in Z-DNA: structure of d(CGCGCA).d(TGCGCG) in two crystal forms.'''<br />
 
-
==Overview==
+
==Cobalt hexammine induced tautameric shift in Z-DNA: structure of d(CGCGCA).d(TGCGCG) in two crystal forms.==
-
We report here the crystal structure of the DNA hexamer duplex d(CGCGCA)*d(TGCGCG) at 1.71 A resolution. The crystals, in orthorhombic space group, were grown in the presence of cobalt hexammine, a known inducer of the left-handed Z form of DNA. The interaction of this ion with the DNA helix results in a change of the adenine base from the common amino tautomeric form to the imino tautomer. Consequently the A:T base pair is disrupted from the normal Watson-Crick base pairing to a 'wobble' like base pairing. This change is accommodated easily within the helix, and the helical parameters are those expected for Z-DNA. When the cobalt hexammine concentration is decreased slightly in the crystallization conditions, the duplex crystallizes in a different, hexagonal space group, with two hexamer duplexes in the asymmetric unit. One of these is situated on a crystallographic 6-fold screw axis, leading to disorder. The tautomeric shift is not observed in this space group. We show that the change in inter-helix interactions that lead to the two different space groups probably arise from the small decrease in ion concentration, and consequently disordered positions for the ion.
+
<StructureSection load='1xam' size='340' side='right'caption='[[1xam]], [[Resolution|resolution]] 1.86&Aring;' scene=''>
-
 
+
== Structural highlights ==
-
==About this Structure==
+
<table><tr><td colspan='2'>[[1xam]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XAM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1XAM FirstGlance]. <br>
-
1XAM is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=NCO:'>NCO</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XAM OCA].
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.86&#8491;</td></tr>
-
 
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NCO:COBALT+HEXAMMINE(III)'>NCO</scene></td></tr>
-
==Reference==
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1xam FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xam OCA], [https://pdbe.org/1xam PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1xam RCSB], [https://www.ebi.ac.uk/pdbsum/1xam PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1xam ProSAT]</span></td></tr>
-
Cobalt hexammine induced tautomeric shift in Z-DNA: the structure of d(CGCGCA)*d(TGCGCG) in two crystal forms., Thiyagarajan S, Rajan SS, Gautham N, Nucleic Acids Res. 2004 Nov 8;32(19):5945-53. Print 2004. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15534365 15534365]
+
</table>
-
[[Category: Protein complex]]
+
__TOC__
-
[[Category: Gautham, N.]]
+
</StructureSection>
-
[[Category: Rajan, S S.]]
+
[[Category: Large Structures]]
-
[[Category: Thiyagarajan, S.]]
+
[[Category: Gautham N]]
-
[[Category: NCO]]
+
[[Category: Rajan SS]]
-
[[Category: double helix]]
+
[[Category: Thiyagarajan S]]
-
[[Category: z-dna]]
+
-
 
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:52:50 2008''
+

Current revision

Cobalt hexammine induced tautameric shift in Z-DNA: structure of d(CGCGCA).d(TGCGCG) in two crystal forms.

PDB ID 1xam

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1xam"
Personal tools