1xbn

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[[Image:1xbn.gif|left|200px]]<br /><applet load="1xbn" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1xbn, resolution 2.50&Aring;" />
 
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'''Crystal structure of a bacterial nitric oxide sensor: an ortholog of mammalian soluble guanylate cyclase heme domain'''<br />
 
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==Overview==
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==Crystal structure of a bacterial nitric oxide sensor: an ortholog of mammalian soluble guanylate cyclase heme domain==
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Nitric oxide (NO) is extremely toxic to Clostridium botulinum, but its molecular targets are unknown. Here, we identify a heme protein sensor (SONO) that displays femtomolar affinity for NO. The crystal structure of the SONO heme domain reveals a previously undescribed fold and a strategically placed tyrosine residue that modulates heme-nitrosyl coordination. Furthermore, the domain architecture of a SONO ortholog cloned from Chlamydomonas reinhardtii indicates that NO signaling through cyclic guanosine monophosphate arose before the origin of multicellular eukaryotes. Our findings have broad implications for understanding bacterial responses to NO, as well as for the activation of mammalian NO-sensitive guanylyl cyclase.
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<StructureSection load='1xbn' size='340' side='right'caption='[[1xbn]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1xbn]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Caldanaerobacter_subterraneus_subsp._tengcongensis_MB4 Caldanaerobacter subterraneus subsp. tengcongensis MB4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XBN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1XBN FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=OXY:OXYGEN+MOLECULE'>OXY</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1xbn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xbn OCA], [https://pdbe.org/1xbn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1xbn RCSB], [https://www.ebi.ac.uk/pdbsum/1xbn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1xbn ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q8RBX6_CALS4 Q8RBX6_CALS4]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/xb/1xbn_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1xbn ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1XBN is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Thermoanaerobacter_tengcongensis Thermoanaerobacter tengcongensis] with <scene name='pdbligand=OXY:'>OXY</scene> and <scene name='pdbligand=HEM:'>HEM</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XBN OCA].
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*[[Chemotaxis protein 3D structures|Chemotaxis protein 3D structures]]
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*[[Methyl-accepting chemotaxis protein|Methyl-accepting chemotaxis protein]]
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==Reference==
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__TOC__
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Femtomolar sensitivity of a NO sensor from Clostridium botulinum., Nioche P, Berka V, Vipond J, Minton N, Tsai AL, Raman CS, Science. 2004 Nov 26;306(5701):1550-3. Epub 2004 Oct 7. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15472039 15472039]
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</StructureSection>
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[[Category: Single protein]]
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[[Category: Caldanaerobacter subterraneus subsp. tengcongensis MB4]]
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[[Category: Thermoanaerobacter tengcongensis]]
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[[Category: Large Structures]]
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[[Category: Nioche, P.]]
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[[Category: Nioche P]]
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[[Category: Raman, C S.]]
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[[Category: Raman CS]]
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[[Category: HEM]]
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[[Category: OXY]]
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[[Category: cgmp]]
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[[Category: heme protein]]
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[[Category: nitric oxide]]
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[[Category: soluble guanylyl cyclase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:53:03 2008''
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Current revision

Crystal structure of a bacterial nitric oxide sensor: an ortholog of mammalian soluble guanylate cyclase heme domain

PDB ID 1xbn

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