1xbt

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[[Image:1xbt.gif|left|200px]]<br /><applet load="1xbt" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1xbt, resolution 2.40&Aring;" />
 
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'''Crystal Structure of Human Thymidine Kinase 1'''<br />
 
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==Overview==
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==Crystal Structure of Human Thymidine Kinase 1==
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Cytosolic thymidine kinase 1, TK1, is a well known cell-cycle-regulated enzyme of importance in nucleotide metabolism as well as an activator of antiviral and anticancer drugs such as 3'-azido-3'-deoxythymidine (AZT). We have now determined the structures of the TK1 family, the human and Ureaplasma urealyticum enzymes, in complex with the feedback inhibitor dTTP. The TK1s have a tetrameric structure in which each subunit contains an alpha/beta-domain that is similar to ATPase domains of members of the RecA structural family and a domain containing a structural zinc. The zinc ion connects beta-structures at the root of a beta-ribbon that forms a stem that widens to a lasso-type loop. The thymidine of dTTP is hydrogen-bonded to main-chain atoms predominantly coming from the lasso loop. This binding is in contrast to other deoxyribonucleoside kinases where specific interactions occur with side chains. The TK1 structure differs fundamentally from the structures of the other deoxyribonucleoside kinases, indicating a different evolutionary origin.
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<StructureSection load='1xbt' size='340' side='right'caption='[[1xbt]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1xbt]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XBT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1XBT FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=TTP:THYMIDINE-5-TRIPHOSPHATE'>TTP</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1xbt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xbt OCA], [https://pdbe.org/1xbt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1xbt RCSB], [https://www.ebi.ac.uk/pdbsum/1xbt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1xbt ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/KITH_HUMAN KITH_HUMAN]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/xb/1xbt_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1xbt ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1XBT is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=ZN:'>ZN</scene>, <scene name='pdbligand=MG:'>MG</scene> and <scene name='pdbligand=TTP:'>TTP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Thymidine_kinase Thymidine kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.21 2.7.1.21] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XBT OCA].
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*[[Thymidine kinase 3D structures|Thymidine kinase 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Structures of thymidine kinase 1 of human and mycoplasmic origin., Welin M, Kosinska U, Mikkelsen NE, Carnrot C, Zhu C, Wang L, Eriksson S, Munch-Petersen B, Eklund H, Proc Natl Acad Sci U S A. 2004 Dec 28;101(52):17970-5. Epub 2004 Dec 20. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15611477 15611477]
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[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Thymidine kinase]]
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[[Category: Carnrot C]]
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[[Category: Carnrot, C.]]
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[[Category: Eklund H]]
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[[Category: Eklund, H.]]
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[[Category: Eriksson S]]
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[[Category: Eriksson, S.]]
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[[Category: Kosinska U]]
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[[Category: Kosinska, U.]]
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[[Category: Mikkelsen NE]]
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[[Category: Mikkelsen, N E.]]
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[[Category: Munch-Petersen B]]
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[[Category: Munch-Petersen, B.]]
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[[Category: Wang L]]
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[[Category: Wang, L.]]
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[[Category: Welin M]]
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[[Category: Welin, M.]]
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[[Category: Zhu C]]
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[[Category: Zhu, C.]]
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[[Category: MG]]
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[[Category: TTP]]
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[[Category: ZN]]
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[[Category: deoxyribonucleoside kinase]]
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[[Category: feedback inhibitor]]
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[[Category: zinc-binding domain]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:53:05 2008''
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Current revision

Crystal Structure of Human Thymidine Kinase 1

PDB ID 1xbt

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