2wag

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[[Image:2wag.png|left|200px]]
 
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{{STRUCTURE_2wag| PDB=2wag | SCENE= }}
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==The Structure of a family 25 Glycosyl hydrolase from Bacillus anthracis.==
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<StructureSection load='2wag' size='340' side='right'caption='[[2wag]], [[Resolution|resolution]] 1.40&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2wag]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_anthracis_str._Ames Bacillus anthracis str. Ames]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WAG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2WAG FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.4&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=15P:POLYETHYLENE+GLYCOL+(N=34)'>15P</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2wag FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wag OCA], [https://pdbe.org/2wag PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2wag RCSB], [https://www.ebi.ac.uk/pdbsum/2wag PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2wag ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/A0A6L7H2K3_BACAN A0A6L7H2K3_BACAN]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/wa/2wag_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2wag ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Lysozymes are found in many of the sequence-based families of glycoside hydrolases (www.cazy.org) where they show considerable structural and mechanistic diversity. Lysozymes from glycoside hydrolase family GH25 adopt a (alpha/beta)(5)(beta)(3)-barrel-like fold with a proposal in the literature that these enzymes act with inversion of anomeric configuration; the lack of a suitable substrate, however, means that no group has successfully demonstrated the configuration of the product. Here we report the 3-D structure of the GH25 enzyme from Bacillus anthracis at 1.4A resolution. We show that the active center is extremely similar to those from glycoside hydrolase families GH18, GH20, GH56, GH84, and GH85 implying that, in the absence of evidence to the contrary, GH25 enzymes also act with net retention of anomeric configuration using the neighboring-group catalytic mechanism that is common to this 'super-family' of enzymes.
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===THE STRUCTURE OF A FAMILY 25 GLYCOSYL HYDROLASE FROM BACILLUS ANTHRACIS.===
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The crystal structure of a family GH25 lysozyme from Bacillus anthracis implies a neighboring-group catalytic mechanism with retention of anomeric configuration.,Martinez-Fleites C, Korczynska JE, Davies GJ, Cope MJ, Turkenburg JP, Taylor EJ Carbohydr Res. 2009 Sep 8;344(13):1753-7. Epub 2009 Jun 6. PMID:19595298<ref>PMID:19595298</ref>
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{{ABSTRACT_PUBMED_19595298}}
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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==About this Structure==
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<div class="pdbe-citations 2wag" style="background-color:#fffaf0;"></div>
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[[2wag]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_anthracis Bacillus anthracis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WAG OCA].
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== References ==
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<references/>
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==Reference==
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__TOC__
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<ref group="xtra">PMID:019595298</ref><references group="xtra"/>
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</StructureSection>
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[[Category: Bacillus anthracis]]
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[[Category: Bacillus anthracis str. Ames]]
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[[Category: Lysozyme]]
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[[Category: Large Structures]]
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[[Category: Cope, M.]]
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[[Category: Cope M]]
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[[Category: Korczynska, J E.]]
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[[Category: Korczynska JE]]
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[[Category: Martinez-Fleites, C.]]
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[[Category: Martinez-Fleites C]]
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[[Category: Taylor, E J.]]
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[[Category: Taylor EJ]]
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[[Category: Turkenburg, J P.]]
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[[Category: Turkenburg JP]]
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[[Category: Bacillus anthracis]]
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[[Category: Gh25]]
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[[Category: Hydrolase]]
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[[Category: Lysin]]
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[[Category: Lysozyme]]
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Current revision

The Structure of a family 25 Glycosyl hydrolase from Bacillus anthracis.

PDB ID 2wag

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