2w83

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[[Image:2w83.png|left|200px]]
 
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{{STRUCTURE_2w83| PDB=2w83 | SCENE= }}
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==Crystal structure of the ARF6 GTPase in complex with a specific effector, JIP4==
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<StructureSection load='2w83' size='340' side='right'caption='[[2w83]], [[Resolution|resolution]] 1.93&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2w83]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2W83 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2W83 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.93&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DIO:1,4-DIETHYLENE+DIOXIDE'>DIO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2w83 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2w83 OCA], [https://pdbe.org/2w83 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2w83 RCSB], [https://www.ebi.ac.uk/pdbsum/2w83 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2w83 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/ARF6_HUMAN ARF6_HUMAN] GTP-binding protein involved in protein trafficking; regulates endocytic recycling and cytoskeleton remodeling. May modulate vesicle budding and uncoating within the Golgi apparatus. Functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. Involved in the regulation of dendritic spine development (By similarity). Contributes to the regulation of dendritic branching and filopodia extension.<ref>PMID:7589240</ref> <ref>PMID:14978216</ref> <ref>PMID:11266366</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/w8/2w83_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2w83 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The JNK-interacting proteins, JIP3 and JIP4, are specific effectors of the small GTP-binding protein ARF6. The interaction of ARF6-GTP with the second leucine zipper (LZII) domains of JIP3/JIP4 regulates the binding of JIPs to kinesin-1 and dynactin. Here, we report the crystal structure of ARF6-GTP bound to the JIP4-LZII at 1.9 A resolution. The complex is a heterotetramer with dyad symmetry arranged in an ARF6-(JIP4)(2)-ARF6 configuration. Comparison of the ARF6-JIP4 interface with the equivalent region of ARF1 shows the structural basis of JIP4's specificity for ARF6. Using site-directed mutagenesis and surface plasmon resonance, we further show that non-conserved residues at the switch region borders are the key structural determinants of JIP4 specificity. A structure-derived model of the association of the ARF6-JIP3/JIP4 complex with membranes shows that the JIP4-LZII coiled-coil should lie along the membrane to prevent steric hindrances, resulting in only one ARF6 molecule bound. Such a heterotrimeric complex gives insights to better understand the ARF6-mediated motor switch regulatory function.
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===CRYSTAL STRUCTURE OF THE ARF6 GTPASE IN COMPLEX WITH A SPECIFIC EFFECTOR, JIP4===
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The structural basis of Arf effector specificity: the crystal structure of ARF6 in a complex with JIP4.,Isabet T, Montagnac G, Regazzoni K, Raynal B, El Khadali F, England P, Franco M, Chavrier P, Houdusse A, Menetrey J EMBO J. 2009 Sep 16;28(18):2835-45. Epub 2009 Jul 30. PMID:19644450<ref>PMID:19644450</ref>
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{{ABSTRACT_PUBMED_19644450}}
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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==About this Structure==
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<div class="pdbe-citations 2w83" style="background-color:#fffaf0;"></div>
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[[2w83]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2W83 OCA].
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== References ==
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<references/>
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==Reference==
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__TOC__
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<ref group="xtra">PMID:019644450</ref><references group="xtra"/>
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Chavrier, P.]]
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[[Category: Large Structures]]
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[[Category: England, P.]]
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[[Category: Chavrier P]]
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[[Category: Franco, M.]]
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[[Category: El Khadali F]]
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[[Category: Houdusse, A.]]
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[[Category: England P]]
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[[Category: Isabet, T.]]
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[[Category: Franco M]]
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[[Category: Khadali, F El.]]
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[[Category: Houdusse A]]
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[[Category: Menetrey, J.]]
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[[Category: Isabet T]]
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[[Category: Montagnac, G.]]
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[[Category: Menetrey J]]
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[[Category: Raynal, B.]]
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[[Category: Montagnac G]]
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[[Category: Regazzoni, K.]]
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[[Category: Raynal B]]
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[[Category: Arf]]
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[[Category: Regazzoni K]]
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[[Category: Coiled-coil]]
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[[Category: Effector]]
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[[Category: Er-golgi transport]]
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[[Category: Golgi apparatus]]
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[[Category: Gtp-binding]]
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[[Category: Gtpase]]
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[[Category: Heterotetramer]]
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[[Category: Lipoprotein]]
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[[Category: Myristate]]
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[[Category: Nucleotide-binding]]
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[[Category: Phosphoprotein]]
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[[Category: Protein transport]]
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[[Category: Transport]]
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Current revision

Crystal structure of the ARF6 GTPase in complex with a specific effector, JIP4

PDB ID 2w83

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