2vpp

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[[Image:2vpp.png|left|200px]]
 
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{{STRUCTURE_2vpp| PDB=2vpp | SCENE= }}
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==Drosophila melanogaster deoxyribonucleoside kinase successfully activates gemcitabine in transduced cancer cell lines==
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<StructureSection load='2vpp' size='340' side='right'caption='[[2vpp]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2vpp]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VPP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2VPP FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GEO:GEMCITABINE'>GEO</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2vpp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vpp OCA], [https://pdbe.org/2vpp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2vpp RCSB], [https://www.ebi.ac.uk/pdbsum/2vpp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2vpp ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/DNK_DROME DNK_DROME] Deoxyribonucleoside kinase that has a broad specificity phosphorylating thymidine, deoxyadenosine, deoxycytidine and deoxyguanosine. Specificity is higher for pyrimidine nucleosides. Several anti-viral and anti-cancer nucleoside analogs are also efficiently phosphorylated.<ref>PMID:10446143</ref> <ref>PMID:10692477</ref> <ref>PMID:16008571</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/vp/2vpp_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2vpp ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Drosophila melanogaster multisubstrate deoxyribonucleoside kinase (Dm-dNK) can additionally sensitize human cancer cell lines towards the anti-cancer drug gemcitabine. We show that this property is based on the Dm-dNK ability to efficiently phosphorylate gemcitabine. The 2.2A resolution structure of Dm-dNK in complex with gemcitabine shows that the residues Tyr70 and Arg105 play a crucial role in the firm positioning of gemcitabine by extra interactions made by the fluoride atoms. This explains why gemcitabine is a good substrate for Dm-dNK.
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===DROSOPHILA MELANOGASTER DEOXYRIBONUCLEOSIDE KINASE SUCCESSFULLY ACTIVATES GEMCITABINE IN TRANSDUCED CANCER CELL LINES===
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Drosophila melanogaster deoxyribonucleoside kinase activates gemcitabine.,Knecht W, Mikkelsen NE, Clausen AR, Willer M, Eklund H, Gojkovic Z, Piskur J Biochem Biophys Res Commun. 2009 May 1;382(2):430-3. Epub 2009 Mar 13. PMID:19285960<ref>PMID:19285960</ref>
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{{ABSTRACT_PUBMED_19285960}}
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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==About this Structure==
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<div class="pdbe-citations 2vpp" style="background-color:#fffaf0;"></div>
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[[2vpp]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VPP OCA].
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== References ==
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<references/>
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==Reference==
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__TOC__
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<ref group="xtra">PMID:019285960</ref><references group="xtra"/>
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</StructureSection>
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[[Category: Deoxynucleoside kinase]]
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[[Category: Drosophila melanogaster]]
[[Category: Drosophila melanogaster]]
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[[Category: Clausen, A.]]
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[[Category: Large Structures]]
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[[Category: Gojkovic, Z.]]
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[[Category: Clausen A]]
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[[Category: Knecht, W.]]
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[[Category: Gojkovic Z]]
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[[Category: Mikkelsen, N E.]]
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[[Category: Knecht W]]
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[[Category: Piskur, J.]]
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[[Category: Mikkelsen NE]]
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[[Category: Willer, M.]]
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[[Category: Piskur J]]
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[[Category: Atp-binding]]
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[[Category: Willer M]]
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[[Category: Cancer]]
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[[Category: Dna synthesis]]
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[[Category: Gemcitabine]]
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[[Category: Gene therapy]]
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[[Category: Kinase]]
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[[Category: Nucleoside analog]]
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[[Category: Nucleotide-binding]]
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[[Category: Phosphoprotein]]
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[[Category: Structure-function relationship]]
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[[Category: Transferase]]
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Current revision

Drosophila melanogaster deoxyribonucleoside kinase successfully activates gemcitabine in transduced cancer cell lines

PDB ID 2vpp

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