2z5l

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[[Image:2z5l.png|left|200px]]
 
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{{STRUCTURE_2z5l| PDB=2z5l | SCENE= }}
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==The first ketoreductase of the tylosin PKS==
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<StructureSection load='2z5l' size='340' side='right'caption='[[2z5l]], [[Resolution|resolution]] 1.95&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2z5l]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_fradiae Streptomyces fradiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Z5L OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2Z5L FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.95&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2z5l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2z5l OCA], [https://pdbe.org/2z5l PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2z5l RCSB], [https://www.ebi.ac.uk/pdbsum/2z5l PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2z5l ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/O33954_STRFR O33954_STRFR]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/z5/2z5l_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2z5l ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Because it controls the majority of polyketide stereocenters, the ketoreductase (KR) is a central target in engineering polyketide synthases (PKSs). To elucidate the mechanisms of stereocontrol, the structure of KR from the first module of the tylosin PKS was determined. A comparison with a recently solved erythromycin KR that operates on the same substrate explains why their products have opposite alpha-substituent chiralities. The structure reveals how polyketides are guided into the active site by key residues in different KR types. There are four types of reductase-competent KRs, each capable of fixing a unique combination of alpha-substituent and beta-hydroxyl group chiralities, as well as two types of reductase-incompetent KRs that control alpha-substituent chirality alone. A protocol to assign how a module will enforce substituent chirality based on its sequence is presented.
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===The first ketoreductase of the tylosin PKS===
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A tylosin ketoreductase reveals how chirality is determined in polyketides.,Keatinge-Clay AT Chem Biol. 2007 Aug;14(8):898-908. PMID:17719489<ref>PMID:17719489</ref>
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{{ABSTRACT_PUBMED_17719489}}
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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==About this Structure==
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<div class="pdbe-citations 2z5l" style="background-color:#fffaf0;"></div>
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[[2z5l]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_fradiae Streptomyces fradiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Z5L OCA].
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== References ==
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<references/>
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==Reference==
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__TOC__
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<ref group="xtra">PMID:017719489</ref><references group="xtra"/>
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Streptomyces fradiae]]
[[Category: Streptomyces fradiae]]
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[[Category: Keatinge-Clay, A T.]]
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[[Category: Keatinge-Clay AT]]
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[[Category: Stroud, R M.]]
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[[Category: Stroud RM]]
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[[Category: Polyketide synthase ketoreductase]]
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[[Category: Rossman fold]]
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[[Category: Short-chain dehydrogenase/reductase]]
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[[Category: Transferase]]
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Current revision

The first ketoreductase of the tylosin PKS

PDB ID 2z5l

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