2qvw
From Proteopedia
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| - | [[Image:2qvw.png|left|200px]] | ||
| - | + | ==Structure of Giardia Dicer refined against twinned data== | |
| + | <StructureSection load='2qvw' size='340' side='right'caption='[[2qvw]], [[Resolution|resolution]] 3.00Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[2qvw]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Giardia_intestinalis Giardia intestinalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QVW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2QVW FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2qvw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2qvw OCA], [https://pdbe.org/2qvw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2qvw RCSB], [https://www.ebi.ac.uk/pdbsum/2qvw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2qvw ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/DCL_GIAIC DCL_GIAIC] Involved in cleaving double-stranded RNA in the RNA interference (RNAi) pathway. It produces 21 to 23 bp dsRNAs (siRNAs) which target the selective destruction of homologous RNAs.<ref>PMID:16410517</ref> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The crystal structure of the enzyme Dicer from Giardia intestinalis was solved to 3.3 A resolution by MAD using crystals belonging to space group P2(1)2(1)2 [Macrae et al. (2006), Science, 311, 195-198]. These crystals were derived from crystals that diffracted X-rays to 3.0 A resolution but were refractory to structure determination because they were twinned. It is shown here that the original Dicer crystals represent an unusual case of perfect pseudo-merohedral twinning of orthorhombic crystals. Before the twinning problem was overcome, it was possible to calculate a low-resolution electron-density map in space group P4(1) that was used to build a partial molecular model. Experimental phases were sufficient to identify heavy-atom sites that indicated space-group inconsistency, leading to identification of the true space group. This information guided the search for different crystallization conditions that yielded untwinned crystals and ultimately a fully interpretable electron-density map. | ||
| - | + | An unusual case of pseudo-merohedral twinning in orthorhombic crystals of Dicer.,MacRae IJ, Doudna JA Acta Crystallogr D Biol Crystallogr. 2007 Sep;63(Pt 9):993-9. Epub 2007, Aug 17. PMID:17704568<ref>PMID:17704568</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | == | + | <div class="pdbe-citations 2qvw" style="background-color:#fffaf0;"></div> |
| - | + | == References == | |
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
[[Category: Giardia intestinalis]] | [[Category: Giardia intestinalis]] | ||
| - | [[Category: | + | [[Category: Large Structures]] |
| - | [[Category: Doudna | + | [[Category: Doudna JA]] |
| - | [[Category: MacRae | + | [[Category: MacRae IJ]] |
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Current revision
Structure of Giardia Dicer refined against twinned data
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