1xnr

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[[Image:1xnr.gif|left|200px]]<br /><applet load="1xnr" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1xnr, resolution 3.10&Aring;" />
 
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'''Crystal Structure of an Inosine-Cytosine Wobble Base Pair in the Context of the Decoding Center'''<br />
 
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==Overview==
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==Crystal Structure of an Inosine-Cytosine Wobble Base Pair in the Context of the Decoding Center==
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Here we report the crystal structures of I.C and I.A wobble base pairs in the context of the ribosomal decoding center, clearly showing that the I.A base pair is of an I(anti).A(anti) conformation, as predicted by Crick. Additionally, the structures enable the observation of changes in the anticodon to allow purine-purine base pairing, the 'widest' base pair geometry allowed in the wobble position.
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<StructureSection load='1xnr' size='340' side='right'caption='[[1xnr]], [[Resolution|resolution]] 3.10&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1xnr]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XNR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1XNR FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.1&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PAR:PAROMOMYCIN'>PAR</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1xnr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xnr OCA], [https://pdbe.org/1xnr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1xnr RCSB], [https://www.ebi.ac.uk/pdbsum/1xnr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1xnr ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RS2_THET8 RS2_THET8] Spans the head-body hinge region of the 30S subunit. Is loosely associated with the 30S subunit.[HAMAP-Rule:MF_00291_B]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/xn/1xnr_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1xnr ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1XNR is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus] with <scene name='pdbligand=PAR:'>PAR</scene>, <scene name='pdbligand=MG:'>MG</scene> and <scene name='pdbligand=ZN:'>ZN</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XNR OCA].
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*[[Ribosomal protein THX 3D structures|Ribosomal protein THX 3D structures]]
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*[[Ribosome 3D structures|Ribosome 3D structures]]
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==Reference==
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*[[Transfer RNA (tRNA)|Transfer RNA (tRNA)]]
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Structure of a purine-purine wobble base pair in the decoding center of the ribosome., Murphy FV 4th, Ramakrishnan V, Nat Struct Mol Biol. 2004 Dec;11(12):1251-2. Epub 2004 Nov 21. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15558050 15558050]
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__TOC__
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[[Category: Protein complex]]
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Thermus thermophilus]]
[[Category: Thermus thermophilus]]
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[[Category: Murphy, F V.]]
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[[Category: Murphy FV]]
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[[Category: Ramakrishnan, V.]]
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[[Category: Ramakrishnan V]]
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[[Category: MG]]
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[[Category: PAR]]
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[[Category: ZN]]
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[[Category: 30s]]
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[[Category: decoding]]
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[[Category: ribosome]]
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[[Category: translation]]
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[[Category: trna modification]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:56:46 2008''
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Crystal Structure of an Inosine-Cytosine Wobble Base Pair in the Context of the Decoding Center

PDB ID 1xnr

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