2vez

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[[Image:2vez.png|left|200px]]
 
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{{STRUCTURE_2vez| PDB=2vez | SCENE= }}
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==AfGNA1 crystal structure complexed with Acetyl-CoA and Glucose-6P gives new insights into catalysis==
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<StructureSection load='2vez' size='340' side='right'caption='[[2vez]], [[Resolution|resolution]] 1.45&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2vez]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Aspergillus_fumigatus Aspergillus fumigatus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VEZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2VEZ FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.45&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACO:ACETYL+COENZYME+*A'>ACO</scene>, <scene name='pdbligand=G6P:ALPHA-D-GLUCOSE-6-PHOSPHATE'>G6P</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2vez FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vez OCA], [https://pdbe.org/2vez PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2vez RCSB], [https://www.ebi.ac.uk/pdbsum/2vez PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2vez ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q4WCU5_ASPFU Q4WCU5_ASPFU]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ve/2vez_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2vez ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Glucosamine-6-phosphate N-acetyltransferase (GNA1) catalyses the N-acetylation of d-glucosamine-6-phosphate (GlcN-6P), using acetyl-CoA as an acetyl donor. The product GlcNAc-6P is an intermediate in the biosynthesis UDP-GlcNAc. GNA1 is part of the GCN5-related acetyl transferase family (GNATs), which employ a wide range of acceptor substrates. GNA1 has been genetically validated as an antifungal drug target. Detailed knowledge of the Michaelis complex and trajectory towards the transition state would facilitate rational design of inhibitors of GNA1 and other GNAT enzymes. Using the pseudo-substrate glucose-6-phosphate (Glc-6P) as a probe with GNA1 crystals, we have trapped the first GNAT (pseudo-)Michaelis complex, providing direct evidence for the nucleophilic attack of the substrate amine, and giving insight into the protonation of the thiolate leaving group.
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===AFGNA1 CRYSTAL STRUCTURE COMPLEXED WITH ACETYL-COA AND GLUCOSE-6P GIVES NEW INSIGHTS INTO CATALYSIS===
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Glucose-6-phosphate as a probe for the glucosamine-6-phosphate N-acetyltransferase Michaelis complex.,Hurtado-Guerrero R, Raimi O, Shepherd S, van Aalten DM FEBS Lett. 2007 Dec 11;581(29):5597-600. Epub 2007 Nov 20. PMID:18005663<ref>PMID:18005663</ref>
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{{ABSTRACT_PUBMED_18005663}}
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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==About this Structure==
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<div class="pdbe-citations 2vez" style="background-color:#fffaf0;"></div>
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[[2vez]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Aspergillus_fumigatus Aspergillus fumigatus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VEZ OCA].
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== References ==
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<references/>
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==Reference==
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__TOC__
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<ref group="xtra">PMID:018005663</ref><references group="xtra"/>
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</StructureSection>
[[Category: Aspergillus fumigatus]]
[[Category: Aspergillus fumigatus]]
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[[Category: Glucosamine-phosphate N-acetyltransferase]]
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[[Category: Large Structures]]
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[[Category: Aalten, D M.F Van.]]
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[[Category: Hurtado-Guerrero R]]
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[[Category: Hurtado-Guerrero, R.]]
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[[Category: Raimi O]]
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[[Category: Raimi, O.]]
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[[Category: Shepherd S]]
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[[Category: Shepherd, S.]]
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[[Category: Van Aalten DMF]]
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[[Category: Acyltransferase]]
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[[Category: Transferase]]
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Current revision

AfGNA1 crystal structure complexed with Acetyl-CoA and Glucose-6P gives new insights into catalysis

PDB ID 2vez

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