1xs5

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[[Image:1xs5.gif|left|200px]]<br /><applet load="1xs5" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1xs5, resolution 1.85&Aring;" />
 
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'''The Crystal Structure of Lipoprotein Tp32 from Treponema pallidum'''<br />
 
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==Overview==
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==The Crystal Structure of Lipoprotein Tp32 from Treponema pallidum==
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A structure-to-function approach was undertaken to gain insights into the potential function of the 32-kDa membrane lipoprotein (Tp32) of Treponema pallidum, the syphilis bacterium. The crystal structure of rTp32 (determined at a resolution of 1.85 A) shows that the organization of rTp32 is similar to other periplasmic ligand-binding proteins (PLBPs), in that it consists of two alpha/beta domains, linked by two crossovers, with a binding pocket between them. In the pocket, a molecule of L-methionine was detected in the electron density map. Residues from both domains interact with the ligand. One of the crossover regions is comprised of a 3(10)-helix, a feature not typical in other ligand-binding proteins. Sequence comparison shows strong similarity to other hypothetical methionine-binding proteins. Together, the data support the notion that rTp32 is a component of a periplasmic methionine uptake transporter system in T. pallidum.
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<StructureSection load='1xs5' size='340' side='right'caption='[[1xs5]], [[Resolution|resolution]] 1.85&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1xs5]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Treponema_pallidum Treponema pallidum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XS5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1XS5 FirstGlance]. <br>
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1XS5 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Treponema_pallidum Treponema pallidum] with <scene name='pdbligand=MET:'>MET</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XS5 OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.85&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MET:METHIONINE'>MET</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1xs5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xs5 OCA], [https://pdbe.org/1xs5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1xs5 RCSB], [https://www.ebi.ac.uk/pdbsum/1xs5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1xs5 ProSAT]</span></td></tr>
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Structural evidence that the 32-kilodalton lipoprotein (Tp32) of Treponema pallidum is an L-methionine-binding protein., Deka RK, Neil L, Hagman KE, Machius M, Tomchick DR, Brautigam CA, Norgard MV, J Biol Chem. 2004 Dec 31;279(53):55644-50. Epub 2004 Oct 15. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15489229 15489229]
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</table>
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[[Category: Single protein]]
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== Function ==
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[https://www.uniprot.org/uniprot/TPN32_TREPA TPN32_TREPA]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/xs/1xs5_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1xs5 ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Treponema pallidum]]
[[Category: Treponema pallidum]]
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[[Category: Brautigam, C A.]]
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[[Category: Brautigam CA]]
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[[Category: Deka, R K.]]
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[[Category: Deka RK]]
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[[Category: Hagman, K E.]]
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[[Category: Hagman KE]]
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[[Category: Machius, M.]]
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[[Category: Machius M]]
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[[Category: Neil, L.]]
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[[Category: Neil L]]
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[[Category: Norgard, M V.]]
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[[Category: Norgard MV]]
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[[Category: Tomchick, D R.]]
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[[Category: Tomchick DR]]
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[[Category: MET]]
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[[Category: lipoprotein]]
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[[Category: methionine]]
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[[Category: periplasmic binding protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:58:08 2008''
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Current revision

The Crystal Structure of Lipoprotein Tp32 from Treponema pallidum

PDB ID 1xs5

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