2x9q

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[[Image:2x9q.png|left|200px]]
 
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{{STRUCTURE_2x9q| PDB=2x9q | SCENE= }}
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==Structure of the Mycobacterium tuberculosis protein, Rv2275, demonstrates that cyclodipeptide synthetases are related to type I tRNA-Synthetases.==
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<StructureSection load='2x9q' size='340' side='right'caption='[[2x9q]], [[Resolution|resolution]] 2.02&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2x9q]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_H37Rv Mycobacterium tuberculosis H37Rv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2X9Q OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2X9Q FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.02&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2x9q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2x9q OCA], [https://pdbe.org/2x9q PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2x9q RCSB], [https://www.ebi.ac.uk/pdbsum/2x9q PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2x9q ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/CDTS_MYCTU CDTS_MYCTU] Involved in the biosynthesis of mycocyclosin. It uses activated amino acids in the form of aminoacyl-tRNAs (aa-tRNAs) as substrates to catalyze the ATP-independent formation of cyclodipeptides which are intermediates in diketopiperazine (DKP) biosynthetic pathways. Catalyzes the formation of cyclo(L-Tyr-L-Tyr) (cYY) from L-tyrosyl-tRNA(Tyr). Can incorporate various nonpolar residues, such as L-phenylalanine, L-leucine, L-tyrosine and L-methionine, and to a much lesser extent L-alanine, into cyclodipeptides. Cyclodipeptides synthesized by Rv2275 always contain L-tyrosine.<ref>PMID:19430487</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/x9/2x9q_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2x9q ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The Mycobacterium tuberculosis enzyme Rv2275 catalyzes the formation of cyclo(L-Tyr-L-Tyr) using two molecules of Tyr-tRNA(Tyr) as substrates. The three-dimensional (3D) structure of Rv2275 was determined to 2.0-A resolution, revealing that Rv2275 is structurally related to the class Ic aminoacyl-tRNA synthetase family of enzymes. Mutagenesis and radioactive labeling suggests a covalent intermediate in which L-tyrosine is transferred from Tyr-tRNA(Tyr) to an active site serine (Ser88) by transesterification with Glu233 serving as a critical base, catalyzing dipeptide bond formation.
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===STRUCTURE OF THE MYCOBACTERIUM TUBERCULOSIS PROTEIN, RV2275, DEMONSTRATES THAT CYCLODIPEPTIDE SYNTHETASES ARE RELATED TO TYPE I TRNA-SYNTHETASES.===
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The structure and mechanism of the Mycobacterium tuberculosis cyclodityrosine synthetase.,Vetting MW, Hegde SS, Blanchard JS Nat Chem Biol. 2010 Sep 19. PMID:20852636<ref>PMID:20852636</ref>
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{{ABSTRACT_PUBMED_20852636}}
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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==About this Structure==
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<div class="pdbe-citations 2x9q" style="background-color:#fffaf0;"></div>
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[[2x9q]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2X9Q OCA].
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== References ==
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<references/>
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==Reference==
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__TOC__
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<ref group="xtra">PMID:020852636</ref><references group="xtra"/>
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</StructureSection>
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[[Category: Mycobacterium tuberculosis]]
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[[Category: Large Structures]]
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[[Category: Blanchard, J S.]]
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[[Category: Mycobacterium tuberculosis H37Rv]]
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[[Category: Hegde, S S.]]
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[[Category: Blanchard JS]]
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[[Category: Vetting, M W.]]
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[[Category: Hegde SS]]
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[[Category: Ligase]]
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[[Category: Vetting MW]]

Current revision

Structure of the Mycobacterium tuberculosis protein, Rv2275, demonstrates that cyclodipeptide synthetases are related to type I tRNA-Synthetases.

PDB ID 2x9q

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