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3bws
From Proteopedia
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| - | [[Image:3bws.png|left|200px]] | ||
| - | + | ==Crystal structure of the leptospiral antigen Lp49== | |
| + | <StructureSection load='3bws' size='340' side='right'caption='[[3bws]], [[Resolution|resolution]] 1.99Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[3bws]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/"leptospira_icteroides"_noguchi_1919 "leptospira icteroides" noguchi 1919]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BWS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3BWS FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | ||
| + | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | ||
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">LIC10793 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=173 "Leptospira icteroides" Noguchi 1919])</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3bws FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3bws OCA], [https://pdbe.org/3bws PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3bws RCSB], [https://www.ebi.ac.uk/pdbsum/3bws PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3bws ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Evolutionary Conservation == | ||
| + | [[Image:Consurf_key_small.gif|200px|right]] | ||
| + | Check<jmol> | ||
| + | <jmolCheckbox> | ||
| + | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bw/3bws_consurf.spt"</scriptWhenChecked> | ||
| + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | ||
| + | <text>to colour the structure by Evolutionary Conservation</text> | ||
| + | </jmolCheckbox> | ||
| + | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3bws ConSurf]. | ||
| + | <div style="clear:both"></div> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Pathogenic Leptospira is the etiological agent of leptospirosis, a life-threatening disease that affects populations worldwide. Currently available vaccines have limited effectiveness and therapeutic interventions are complicated by the difficulty in making an early diagnosis of leptospirosis. The genome of Leptospira interrogans was recently sequenced and comparative genomic analysis contributed to the identification of surface antigens, potential candidates for development of new vaccines and serodiagnosis. Lp49 is a membrane-associated protein recognized by antibodies present in sera from early and convalescent phases of leptospirosis patients. Its crystal structure was determined by single-wavelength anomalous diffraction using selenomethionine-labelled crystals and refined at 2.0 A resolution. Lp49 is composed of two domains and belongs to the all-beta-proteins class. The N-terminal domain folds in an immunoglobulin-like beta-sandwich structure, whereas the C-terminal domain presents a seven-bladed beta-propeller fold. Structural analysis of Lp49 indicates putative protein-protein binding sites, suggesting a role in Leptospira-host interaction. This is the first crystal structure of a leptospiral antigen described to date. | ||
| - | + | The leptospiral antigen Lp49 is a two-domain protein with putative protein binding function.,Giuseppe PO, Neves FO, Nascimento AL, Guimaraes BG J Struct Biol. 2008 Jul;163(1):53-60. Epub 2008 Apr 14. PMID:18508281<ref>PMID:18508281</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | <div class="pdbe-citations 3bws" style="background-color:#fffaf0;"></div> | |
| - | + | == References == | |
| - | + | <references/> | |
| - | == | + | __TOC__ |
| - | < | + | </StructureSection> |
| - | [[Category: Leptospira | + | [[Category: Leptospira icteroides noguchi 1919]] |
| - | [[Category: Giuseppe, P O | + | [[Category: Large Structures]] |
| - | [[Category: Guimaraes, B G | + | [[Category: Giuseppe, P O]] |
| - | [[Category: Nascimento, A L.T O | + | [[Category: Guimaraes, B G]] |
| - | [[Category: Neves, F O | + | [[Category: Nascimento, A L.T O]] |
| + | [[Category: Neves, F O]] | ||
[[Category: 7-bladed beta propeller]] | [[Category: 7-bladed beta propeller]] | ||
[[Category: Immunoglobulin-like]] | [[Category: Immunoglobulin-like]] | ||
[[Category: Two-domain]] | [[Category: Two-domain]] | ||
[[Category: Unknown function]] | [[Category: Unknown function]] | ||
Current revision
Crystal structure of the leptospiral antigen Lp49
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