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1xvx

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[[Image:1xvx.gif|left|200px]]<br /><applet load="1xvx" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1xvx, resolution 1.53&Aring;" />
 
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'''Crystal Structure of iron-loaded Yersinia enterocolitica YfuA'''<br />
 
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==Overview==
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==Crystal Structure of iron-loaded Yersinia enterocolitica YfuA==
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The uptake of the element iron is vital for the survival of most organisms. Numerous pathogenic Gram-negative bacteria utilize a periplasm-to-cytosol ATP-binding cassette transport pathway to transport this essential atom in to the cell. In this study, we investigated the Yersinia enterocolitica (YfuA) and Serratia marcescens (SfuA) iron-binding periplasmic proteins. We have determined the 1.8-angstroms structures of iron-loaded (YfuA) and iron-free (SfuA) forms of this class of proteins. Although the sequence of these proteins varies considerably from the other members of the transferrin structural superfamily, they adopt the same three-dimensional fold. The iron-loaded YfuA structure illustrates the unique nature of this new class of proteins in that they are able to octahedrally coordinate the ferric ion in the absence of a bound anion. The iron-free SfuA structure contains a bound citrate anion in the iron-binding cleft that tethers the N- and C-terminal domains of the apo protein and stabilizes the partially open structure.
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<StructureSection load='1xvx' size='340' side='right'caption='[[1xvx]], [[Resolution|resolution]] 1.53&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1xvx]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Yersinia_enterocolitica Yersinia enterocolitica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XVX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1XVX FirstGlance]. <br>
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1XVX is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Yersinia_enterocolitica Yersinia enterocolitica] with <scene name='pdbligand=FE:'>FE</scene>, <scene name='pdbligand=ZN:'>ZN</scene>, <scene name='pdbligand=CO3:'>CO3</scene> and <scene name='pdbligand=EDO:'>EDO</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XVX OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.53&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CO3:CARBONATE+ION'>CO3</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1xvx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xvx OCA], [https://pdbe.org/1xvx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1xvx RCSB], [https://www.ebi.ac.uk/pdbsum/1xvx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1xvx ProSAT]</span></td></tr>
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Novel anion-independent iron coordination by members of a third class of bacterial periplasmic ferric ion-binding proteins., Shouldice SR, McRee DE, Dougan DR, Tari LW, Schryvers AB, J Biol Chem. 2005 Feb 18;280(7):5820-7. Epub 2004 Dec 2. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15576371 15576371]
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</table>
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[[Category: Single protein]]
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== Function ==
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[https://www.uniprot.org/uniprot/A1JLH5_YERE8 A1JLH5_YERE8]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/xv/1xvx_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1xvx ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Yersinia enterocolitica]]
[[Category: Yersinia enterocolitica]]
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[[Category: Dougan, D R.]]
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[[Category: Dougan DR]]
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[[Category: McRee, D E.]]
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[[Category: McRee DE]]
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[[Category: Schryvers, A B.]]
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[[Category: Schryvers AB]]
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[[Category: Shouldice, S R.]]
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[[Category: Shouldice SR]]
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[[Category: Tari, L W.]]
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[[Category: Tari LW]]
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[[Category: CO3]]
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[[Category: EDO]]
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[[Category: FE]]
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[[Category: ZN]]
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[[Category: periplasmic iron binding protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:59:14 2008''
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Current revision

Crystal Structure of iron-loaded Yersinia enterocolitica YfuA

PDB ID 1xvx

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