3bb1

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[[Image:3bb1.png|left|200px]]
 
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{{STRUCTURE_3bb1| PDB=3bb1 | SCENE= }}
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==Crystal structure of Toc34 from Pisum sativum in complex with Mg2+ and GMPPNP==
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<StructureSection load='3bb1' size='340' side='right'caption='[[3bb1]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3bb1]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Pisum_sativum Pisum sativum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BB1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3BB1 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GNP:PHOSPHOAMINOPHOSPHONIC+ACID-GUANYLATE+ESTER'>GNP</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3bb1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3bb1 OCA], [https://pdbe.org/3bb1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3bb1 RCSB], [https://www.ebi.ac.uk/pdbsum/3bb1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3bb1 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/TOC34_PEA TOC34_PEA] GTPase involved in protein precursor import into chloroplasts. Seems to recognize chloroplast-destined precursor proteins and regulate their presentation to the translocation channel through GTP hydrolysis.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bb/3bb1_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3bb1 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Transport of precursor proteins across chloroplast membranes involves the GTPases Toc33/34 and Toc159 at the outer chloroplast envelope. The small GTPase Toc33/34 can homodimerize, but the regulation of this interaction has remained elusive. We show that dimerization is independent of nucleotide loading state, based on crystal structures of dimeric Pisum sativum Toc34 and monomeric Arabidopsis thaliana Toc33. An arginine residue is--in the dimer--positioned to resemble a GAP arginine finger. However, GTPase activation by dimerization is sparse and active site features do not explain catalysis, suggesting that the homodimer requires an additional factor as coGAP. Access to the catalytic center and an unusual switch I movement in the dimeric structure support this finding. Potential binding sites for interactions within the Toc translocon or with precursor proteins can be derived from the structures.
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===Crystal structure of Toc34 from Pisum sativum in complex with Mg2+ and GMPPNP===
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The GTPase cycle of the chloroplast import receptors Toc33/Toc34: implications from monomeric and dimeric structures.,Koenig P, Oreb M, Hofle A, Kaltofen S, Rippe K, Sinning I, Schleiff E, Tews I Structure. 2008 Apr;16(4):585-96. PMID:18400179<ref>PMID:18400179</ref>
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{{ABSTRACT_PUBMED_18400179}}
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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==About this Structure==
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<div class="pdbe-citations 3bb1" style="background-color:#fffaf0;"></div>
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[[3bb1]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Pisum_sativum Pisum sativum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BB1 OCA].
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== References ==
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<references/>
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==Reference==
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__TOC__
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<ref group="xtra">PMID:018400179</ref><references group="xtra"/>
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Pisum sativum]]
[[Category: Pisum sativum]]
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[[Category: Koenig, P.]]
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[[Category: Koenig P]]
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[[Category: Schleiff, E.]]
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[[Category: Schleiff E]]
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[[Category: Sinning, I.]]
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[[Category: Sinning I]]
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[[Category: Tews, I.]]
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[[Category: Tews I]]
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[[Category: Chloroplast import]]
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[[Category: Gtp-binding]]
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[[Category: Gtpase domain]]
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[[Category: Hydrolase]]
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[[Category: Membrane]]
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[[Category: Nucleotide-binding]]
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[[Category: Outer membrane]]
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[[Category: Protein transport]]
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[[Category: Rossman fold]]
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[[Category: Transmembrane]]
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[[Category: Transport]]
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Current revision

Crystal structure of Toc34 from Pisum sativum in complex with Mg2+ and GMPPNP

PDB ID 3bb1

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