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1xyz

From Proteopedia

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[[Image:1xyz.gif|left|200px]]<br /><applet load="1xyz" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1xyz, resolution 1.4&Aring;" />
 
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'''A COMMON PROTEIN FOLD AND SIMILAR ACTIVE SITE IN TWO DISTINCT FAMILIES OF BETA-GLYCANASES'''<br />
 
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==Overview==
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==A COMMON PROTEIN FOLD AND SIMILAR ACTIVE SITE IN TWO DISTINCT FAMILIES OF BETA-GLYCANASES==
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The structure of Clostridium thermocellum endoglucanase CelC, a member of the largest cellulase family (family A), has been determined at 2.15 A resolution. The protein folds into an (alpha/beta)8 barrel, with a deep active-site cleft generated by the insertion of a helical subdomain. The structure of the catalytic core of xylanase XynZ, which belongs to xylanase family F, has been determined at 1.4 A resolution. In spite of significant differences in substrate specificity and structure (including the absence of the helical subdomain), the general polypeptide folding pattern, architecture of the active site and catalytic mechanism of XynZ and CelC are similar, suggesting a common evolutionary origin.
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<StructureSection load='1xyz' size='340' side='right'caption='[[1xyz]], [[Resolution|resolution]] 1.40&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1xyz]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Acetivibrio_thermocellus Acetivibrio thermocellus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XYZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1XYZ FirstGlance]. <br>
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1XYZ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Clostridium_thermocellum Clostridium thermocellum]. Active as [http://en.wikipedia.org/wiki/Endo-1,4-beta-xylanase Endo-1,4-beta-xylanase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.8 3.2.1.8] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XYZ OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.4&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1xyz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xyz OCA], [https://pdbe.org/1xyz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1xyz RCSB], [https://www.ebi.ac.uk/pdbsum/1xyz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1xyz ProSAT]</span></td></tr>
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==Reference==
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</table>
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A common protein fold and similar active site in two distinct families of beta-glycanases., Dominguez R, Souchon H, Spinelli S, Dauter Z, Wilson KS, Chauvaux S, Beguin P, Alzari PM, Nat Struct Biol. 1995 Jul;2(7):569-76. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=7664125 7664125]
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== Function ==
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[[Category: Clostridium thermocellum]]
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[https://www.uniprot.org/uniprot/XYNZ_ACET2 XYNZ_ACET2]
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[[Category: Endo-1,4-beta-xylanase]]
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== Evolutionary Conservation ==
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[[Category: Single protein]]
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[[Image:Consurf_key_small.gif|200px|right]]
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[[Category: Alzari, P M.]]
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Check<jmol>
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[[Category: Dominguez, R.]]
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<jmolCheckbox>
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[[Category: Spinelli, S.]]
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/xy/1xyz_consurf.spt"</scriptWhenChecked>
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[[Category: clostridium thermocellum]]
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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[[Category: family f/10 of glycosyl hydrolases]]
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<text>to colour the structure by Evolutionary Conservation</text>
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[[Category: glycosyl hydrolase]]
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</jmolCheckbox>
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[[Category: xylanase]]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1xyz ConSurf].
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<div style="clear:both"></div>
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:00:10 2008''
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__TOC__
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</StructureSection>
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[[Category: Acetivibrio thermocellus]]
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[[Category: Large Structures]]
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[[Category: Alzari PM]]
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[[Category: Dominguez R]]
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[[Category: Spinelli S]]

Current revision

A COMMON PROTEIN FOLD AND SIMILAR ACTIVE SITE IN TWO DISTINCT FAMILIES OF BETA-GLYCANASES

PDB ID 1xyz

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