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2wdf

From Proteopedia

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[[Image:2wdf.png|left|200px]]
 
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{{STRUCTURE_2wdf| PDB=2wdf | SCENE= }}
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==Termus thermophilus Sulfate thiohydrolase SoxB==
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<StructureSection load='2wdf' size='340' side='right'caption='[[2wdf]], [[Resolution|resolution]] 2.08&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2wdf]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_thermophilus_HB27 Thermus thermophilus HB27]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WDF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2WDF FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.08&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=TBU:TERTIARY-BUTYL+ALCOHOL'>TBU</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2wdf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wdf OCA], [https://pdbe.org/2wdf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2wdf RCSB], [https://www.ebi.ac.uk/pdbsum/2wdf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2wdf ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q72IT0_THET2 Q72IT0_THET2]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/wd/2wdf_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2wdf ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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SoxB is an essential component of the bacterial Sox sulfur oxidation pathway. SoxB contains a di-manganese(II) site and is proposed to catalyze the release of sulfate from a protein-bound cysteine S-thiosulfonate. A direct assay for SoxB activity is described. The structure of recombinant Thermus thermophilus SoxB was determined by x-ray crystallography to a resolution of 1.5 A. Structures were also determined for SoxB in complex with the substrate analogue thiosulfate and in complex with the product sulfate. A mechanistic model for SoxB is proposed based on these structures.
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===TERMUS THERMOPHILUS SULFATE THIOHYDROLASE SOXB===
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Mechanism for the hydrolysis of a sulfur-sulfur bond based on the crystal structure of the thiosulfohydrolase SoxB.,Sauve V, Roversi P, Leath KJ, Garman EF, Antrobus R, Lea SM, Berks BC J Biol Chem. 2009 Aug 7;284(32):21707-18. Epub 2009 Jun 16. PMID:19535341<ref>PMID:19535341</ref>
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{{ABSTRACT_PUBMED_19535341}}
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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==About this Structure==
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<div class="pdbe-citations 2wdf" style="background-color:#fffaf0;"></div>
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[[2wdf]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WDF OCA].
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== References ==
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<references/>
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==Reference==
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__TOC__
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<ref group="xtra">PMID:019535341</ref><references group="xtra"/>
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</StructureSection>
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[[Category: Hydrolase]]
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[[Category: Large Structures]]
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[[Category: Thermus thermophilus]]
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[[Category: Thermus thermophilus HB27]]
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[[Category: Antrobus, R.]]
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[[Category: Antrobus R]]
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[[Category: Berks, B C.]]
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[[Category: Berks BC]]
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[[Category: Garman, E F.]]
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[[Category: Garman EF]]
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[[Category: Lea, S M.]]
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[[Category: Lea SM]]
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[[Category: Leath, K J.]]
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[[Category: Leath KJ]]
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[[Category: Roversi, P.]]
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[[Category: Roversi P]]
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[[Category: Sauve, V.]]
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[[Category: Sauve V]]
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[[Category: Cys s-thiosulfonate]]
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[[Category: Hydrolase]]
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[[Category: Sox]]
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[[Category: Soxb]]
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[[Category: Sulfur oxidation pathway]]
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[[Category: Sulfur-sulfur hydrolysis]]
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Current revision

Termus thermophilus Sulfate thiohydrolase SoxB

PDB ID 2wdf

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