2vbe

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[[Image:2vbe.png|left|200px]]
 
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{{STRUCTURE_2vbe| PDB=2vbe | SCENE= }}
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==Tailspike protein of bacteriophage Sf6==
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<StructureSection load='2vbe' size='340' side='right'caption='[[2vbe]], [[Resolution|resolution]] 1.98&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2vbe]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Shigella_virus_Sf6 Shigella virus Sf6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VBE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2VBE FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.98&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2vbe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vbe OCA], [https://pdbe.org/2vbe PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2vbe RCSB], [https://www.ebi.ac.uk/pdbsum/2vbe PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2vbe ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/FIBER_BPSFV FIBER_BPSFV] Non-covalently bound to the neck of the phage capsid and mediating attachment of the viral particle to host cell-surface polysaccharide. It displays endorhamnosidase enzymatic activity, hydrolyzing the alpha-1,3-O-glycosidic linkage between rhamnose and galactose of the O-antigen polysaccharide.<ref>PMID:18462681</ref> <ref>PMID:12424253</ref> <ref>PMID:6284</ref>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Sf6 belongs to the Podoviridae family of temperate bacteriophages that infect gram-negative bacteria by insertion of their double-stranded DNA. They attach to their hosts specifically via their tailspike proteins. The 1.25 A crystal structure of Shigella phage Sf6 tailspike protein (Sf6 TSP) reveals a conserved architecture with a central, right-handed beta helix. In the trimer of Sf6 TSP, the parallel beta helices form a left-handed, coiled-beta coil with a pitch of 340 A. The C-terminal domain consists of a beta sandwich reminiscent of viral capsid proteins. Further crystallographic and biochemical analyses show a Shigella cell wall O-antigen fragment to bind to an endorhamnosidase active site located between two beta-helix subunits each anchoring one catalytic carboxylate. The functionally and structurally related bacteriophage, P22 TSP, lacks sequence identity with Sf6 TSP and has its active sites on single subunits. Sf6 TSP may serve as an example for the evolution of different host specificities on a similar general architecture.
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===TAILSPIKE PROTEIN OF BACTERIOPHAGE SF6===
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An intersubunit active site between supercoiled parallel beta helices in the trimeric tailspike endorhamnosidase of Shigella flexneri Phage Sf6.,Muller JJ, Barbirz S, Heinle K, Freiberg A, Seckler R, Heinemann U Structure. 2008 May;16(5):766-75. PMID:18462681<ref>PMID:18462681</ref>
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{{ABSTRACT_PUBMED_18462681}}
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 2vbe" style="background-color:#fffaf0;"></div>
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==About this Structure==
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==See Also==
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[[2vbe]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Shigella_phage_sf6 Shigella phage sf6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VBE OCA].
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*[[Tailspike protein 3D structures|Tailspike protein 3D structures]]
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:018462681</ref><references group="xtra"/>
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__TOC__
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[[Category: Shigella phage sf6]]
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</StructureSection>
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[[Category: Barbirz, S.]]
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[[Category: Large Structures]]
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[[Category: Freiberg, A.]]
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[[Category: Shigella virus Sf6]]
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[[Category: Heinemann, U.]]
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[[Category: Barbirz S]]
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[[Category: Mueller, J J.]]
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[[Category: Freiberg A]]
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[[Category: Seckler, R.]]
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[[Category: Heinemann U]]
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[[Category: Oligomeric right-handed parallel beta-helix]]
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[[Category: Mueller JJ]]
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[[Category: Tailspike]]
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[[Category: Seckler R]]
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[[Category: Viral adhesion protein]]
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[[Category: Viral protein]]
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Current revision

Tailspike protein of bacteriophage Sf6

PDB ID 2vbe

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