3cvr

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[[Image:3cvr.png|left|200px]]
 
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{{STRUCTURE_3cvr| PDB=3cvr | SCENE= }}
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==Crystal structure of the full length IpaH3==
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<StructureSection load='3cvr' size='340' side='right'caption='[[3cvr]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3cvr]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Shigella_flexneri_2a_str._301 Shigella flexneri 2a str. 301]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CVR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3CVR FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3cvr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3cvr OCA], [https://pdbe.org/3cvr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3cvr RCSB], [https://www.ebi.ac.uk/pdbsum/3cvr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3cvr ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/IPA3_SHIFL IPA3_SHIFL] Effector proteins function to alter host cell physiology and promote bacterial survival in host tissues. This protein is an E3 ubiquitin ligase that interferes with host's ubiquitination pathway. Synthesizes a 'Lys-48'-linked ubiquitin chain, which requires non-covalent binding between ubiquitin and the host ubiquitin-conjugating enzyme UBE2D1.<ref>PMID:18997779</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cv/3cvr_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3cvr ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Bacterial pathogens have evolved effector proteins with ubiquitin E3 ligase activities through structural mimicking. Here we report the crystal structure of the Shigella flexneri type III effector IpaH3, a member of the leucine-rich repeat (LRR)-containing bacterial E3 family. The LRR domain is structurally similar to Yersinia pestis YopM and potentially binds to substrates. The structure of the C-terminal E3 domain differs from the typical RING- and HECT-type E3s. IpaH3 synthesizes a Lys48-linked ubiquitin chain, and the reaction requires noncovalent binding between ubiquitin and a specific E2, UbcH5. Free ubiquitin serves as an acceptor for IpaH3-catalyzed ubiquitin transfer. Cys363 within a conserved CXD motif acts as a nucleophile to catalyze ubiquitin transfer through a transthiolation reaction. The D365N mutant is devoid of E3 activities but turns into a potent ubiquitin-E2 thioesterase. Our analysis establishes a structurally and mechanistically distinct class of ubiquitin ligases found exclusively in pathogenic or symbiotic bacteria.
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===Crystal structure of the full length IpaH3===
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Structure of a Shigella effector reveals a new class of ubiquitin ligases.,Zhu Y, Li H, Hu L, Wang J, Zhou Y, Pang Z, Liu L, Shao F Nat Struct Mol Biol. 2008 Dec;15(12):1302-8. Epub 2008 Nov 9. PMID:18997779<ref>PMID:18997779</ref>
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{{ABSTRACT_PUBMED_18997779}}
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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==About this Structure==
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<div class="pdbe-citations 3cvr" style="background-color:#fffaf0;"></div>
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[[3cvr]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Shigella_flexneri_2a Shigella flexneri 2a]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CVR OCA].
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== References ==
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<references/>
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==Reference==
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__TOC__
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<ref group="xtra">PMID:018997779</ref><references group="xtra"/>
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</StructureSection>
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[[Category: Shigella flexneri 2a]]
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[[Category: Large Structures]]
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[[Category: Shao, F.]]
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[[Category: Shigella flexneri 2a str. 301]]
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[[Category: Zhu, Y.]]
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[[Category: Shao F]]
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[[Category: Leucine rich repeat and alpha fold]]
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[[Category: Zhu Y]]
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[[Category: Ligase]]
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Current revision

Crystal structure of the full length IpaH3

PDB ID 3cvr

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