2z7g

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[[Image:2z7g.png|left|200px]]
 
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{{STRUCTURE_2z7g| PDB=2z7g | SCENE= }}
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==Crystal structure of adenosine deaminase ligated with EHNA==
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<StructureSection load='2z7g' size='340' side='right'caption='[[2z7g]], [[Resolution|resolution]] 2.52&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2z7g]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Z7G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2Z7G FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.52&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EH9:(2S,3R)-3-(6-AMINO-9H-PURIN-9-YL)NONAN-2-OL'>EH9</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2z7g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2z7g OCA], [https://pdbe.org/2z7g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2z7g RCSB], [https://www.ebi.ac.uk/pdbsum/2z7g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2z7g ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/ADA_BOVIN ADA_BOVIN] Catalyzes the hydrolytic deamination of adenosine and 2-deoxyadenosine. Plays an important role in purine metabolism and in adenosine homeostasis. Modulates signaling by extracellular adenosine, and so contributes indirectly to cellular signaling events. Acts as a positive regulator of T-cell coactivation, by binding DPP4. Its interaction with DPP4 regulates lymphocyte-epithelial cell adhesion (By similarity).
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/z7/2z7g_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2z7g ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Adenosine deaminase (ADA) perpetuates chronic inflammation by degrading extracellular adenosine which is toxic for lymphocytes. ADA has two distinct conformations: open form and closed form. From the crystal structures with various ligands, the non-nucleoside type inhibitors bind to the active site occupying the critical water-binding-position and sustain the open form of apo-ADA. In contrast, substrate mimics do not occupy the critical position, and induce the large conformational change to the closed form. However, it is difficult to predict the binding of (+)-erythro-9-(2-hydroxy-3-nonyl)adenine (EHNA), as it possesses characteristic parts of both the substrate and the non-nucleoside inhibitors. The crystal structure shows that EHNA binds to the open form through a novel recognition of the adenine base accompanying conformational change from the closed form of the PR-ADA complex in crystalline state.
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===Crystal structure of adenosine deaminase ligated with EHNA===
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Conformational change of adenosine deaminase during ligand-exchange in a crystal.,Kinoshita T, Tada T, Nakanishi I Biochem Biophys Res Commun. 2008 Aug 15;373(1):53-7. Epub 2008 Jun 10. PMID:18549808<ref>PMID:18549808</ref>
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{{ABSTRACT_PUBMED_18549808}}
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 2z7g" style="background-color:#fffaf0;"></div>
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==About this Structure==
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==See Also==
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[[2z7g]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Z7G OCA].
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*[[Adenosine deaminase 3D structures|Adenosine deaminase 3D structures]]
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:018549808</ref><references group="xtra"/>
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__TOC__
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[[Category: Adenosine deaminase]]
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</StructureSection>
[[Category: Bos taurus]]
[[Category: Bos taurus]]
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[[Category: Kinoshita, T.]]
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[[Category: Large Structures]]
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[[Category: Beta barrel]]
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[[Category: Kinoshita T]]
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[[Category: Hydrolase]]
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[[Category: Nucleotide metabolism]]
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[[Category: Protein-inhibitor complex]]
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Current revision

Crystal structure of adenosine deaminase ligated with EHNA

PDB ID 2z7g

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