2zds
From Proteopedia
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| - | [[Image:2zds.png|left|200px]] | ||
| - | + | ==Crystal Structure of SCO6571 from Streptomyces coelicolor A3(2)== | |
| + | <StructureSection load='2zds' size='340' side='right'caption='[[2zds]], [[Resolution|resolution]] 2.30Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[2zds]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/"actinomyces_coelicolor"_(muller_1908)_lieske_1921 "actinomyces coelicolor" (muller 1908) lieske 1921]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZDS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2ZDS FirstGlance]. <br> | ||
| + | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | ||
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">sco6571 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1902 "Actinomyces coelicolor" (Muller 1908) Lieske 1921])</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2zds FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zds OCA], [https://pdbe.org/2zds PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2zds RCSB], [https://www.ebi.ac.uk/pdbsum/2zds PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2zds ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Evolutionary Conservation == | ||
| + | [[Image:Consurf_key_small.gif|200px|right]] | ||
| + | Check<jmol> | ||
| + | <jmolCheckbox> | ||
| + | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/zd/2zds_consurf.spt"</scriptWhenChecked> | ||
| + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | ||
| + | <text>to colour the structure by Evolutionary Conservation</text> | ||
| + | </jmolCheckbox> | ||
| + | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2zds ConSurf]. | ||
| + | <div style="clear:both"></div> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | SCO6571 protein from Streptomyces coelicolor A3(2) was overexpressed and purified using Rhodococcus erythropolis as an expressing host. Crystals of selenomethionine-substituted SCO6571 have been obtained by vapor diffusion method. SCO6571 crystals diffract to 2.3 A and were found to belong to the orthorhombic space group P2(1)2(1)2(1) with unit cell parameters a = 84.5, b = 171.6, c = 184.8 A. Six molecules in the asymmetric unit give a crystal volume per protein mass (V(M)) of 2.97 A (3) Da(-1) and solvent content of 58.6 %. The structure was solved by the single wavelength anomalous diffraction (SAD) method. SCO6571 is a TIM-barrel fold protein that assembles into a hexameric molecule with D(3) symmetry. | ||
| - | + | Crystal structure of SCO6571 from Streptomyces coelicolor A3(2).,Begum P, Sakai N, Hayashi T, Gao YG, Tamura T, Watanabe N, Yao M, Tanaka I Protein Pept Lett. 2008;15(7):709-12. PMID:18782066<ref>PMID:18782066</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | == | + | <div class="pdbe-citations 2zds" style="background-color:#fffaf0;"></div> |
| - | + | == References == | |
| - | [[Category: | + | <references/> |
| - | [[Category: Begum, P | + | __TOC__ |
| - | [[Category: Gao, Y G | + | </StructureSection> |
| - | [[Category: Sakai, N | + | [[Category: Large Structures]] |
| - | [[Category: Tanaka, I | + | [[Category: Begum, P]] |
| - | [[Category: Watanabe, N | + | [[Category: Gao, Y G]] |
| - | [[Category: Yao, M | + | [[Category: Sakai, N]] |
| + | [[Category: Tanaka, I]] | ||
| + | [[Category: Watanabe, N]] | ||
| + | [[Category: Yao, M]] | ||
[[Category: Dna binding protein]] | [[Category: Dna binding protein]] | ||
[[Category: Dna-binding]] | [[Category: Dna-binding]] | ||
Current revision
Crystal Structure of SCO6571 from Streptomyces coelicolor A3(2)
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