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1h80

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[[Image:1h80.gif|left|200px]]<br />
 
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<applet load="1h80" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1h80, resolution 1.60&Aring;" />
 
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'''1,3-ALPHA-1,4-BETA-D-GALACTOSE-4-SULFATE-3,6-ANHYDRO-D-GALACTOSE-2-SULFATE 4 GALACTOHYDROLASE'''<br />
 
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==Overview==
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==1,3-ALPHA-1,4-BETA-D-GALACTOSE-4-SULFATE- 3,6-ANHYDRO-D-GALACTOSE-2-SULFATE 4 GALACTOHYDROLASE==
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Carrageenans are gel-forming hydrocolloids extracted from the cell walls, of marine red algae. They consist of d-galactose residues bound by, alternate alpha(1--&gt;3) and beta(1--&gt;4) linkages and substituted by one, (kappa-carrageenan), two (iota-carrageenan), or three (lambda-carrageenan), sulfate-ester groups per disaccharide repeating unit. Both the kappa- and, iota-carrageenan chains adopt ordered conformations leading to the, formation of highly ordered aggregates of double-stranded helices. Several, kappa-carrageenases and iota-carrageenases have been cloned from marine, bacteria. Kappa-carrageenases belong to family 16 of the glycoside, hydrolases, which essentially encompasses polysaccharidases specialized in, the hydrolysis of the neutral polysaccharides such as agarose, ... [[http://ispc.weizmann.ac.il/pmbin/getpm?11493601 (full description)]]
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<StructureSection load='1h80' size='340' side='right'caption='[[1h80]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1h80]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Alteromonas_macleodii Alteromonas macleodii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1H80 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1H80 FirstGlance]. <br>
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1H80 is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Alteromonas_sp._atcc_43554 Alteromonas sp. atcc 43554]] with CA, CL, NA and GOL as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/Iota-carrageenase Iota-carrageenase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.157 3.2.1.157]]. Structure known Active Site: AC1. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1H80 OCA]].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1h80 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1h80 OCA], [https://pdbe.org/1h80 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1h80 RCSB], [https://www.ebi.ac.uk/pdbsum/1h80 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1h80 ProSAT]</span></td></tr>
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The iota-carrageenase of Alteromonas fortis. A beta-helix fold-containing enzyme for the degradation of a highly polyanionic polysaccharide., Michel G, Chantalat L, Fanchon E, Henrissat B, Kloareg B, Dideberg O, J Biol Chem. 2001 Oct 26;276(43):40202-9. Epub 2001 Aug 7. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11493601 11493601]
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</table>
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[[Category: Alteromonas sp. atcc 43554]]
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== Function ==
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[[Category: Iota-carrageenase]]
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[https://www.uniprot.org/uniprot/CGIA_ALTMA CGIA_ALTMA] Hydrolyzes iota-carrageenans, sulfated 1,3-alpha-1,4-beta galactans from red algal cell walls, with an inversion of anomeric configuration. Also active against hybrid iota-/nu-carrageenan, not active against kappa- or lambda-carrageenans.[UniProtKB:Q9F284]<ref>PMID:10934194</ref> <ref>PMID:20227066</ref>
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[[Category: Single protein]]
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== Evolutionary Conservation ==
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[[Category: Chantalat, L.]]
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[[Image:Consurf_key_small.gif|200px|right]]
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[[Category: Dideberg, O.]]
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Check<jmol>
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[[Category: Michel, G.]]
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<jmolCheckbox>
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[[Category: CA]]
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/h8/1h80_consurf.spt"</scriptWhenChecked>
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[[Category: CL]]
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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[[Category: GOL]]
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<text>to colour the structure by Evolutionary Conservation</text>
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[[Category: NA]]
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</jmolCheckbox>
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[[Category: hydrolase]]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1h80 ConSurf].
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[[Category: iota-carrageenan double helix degradation]]
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<div style="clear:both"></div>
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== References ==
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 15:31:44 2007''
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Alteromonas macleodii]]
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[[Category: Large Structures]]
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[[Category: Chantalat L]]
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[[Category: Dideberg O]]
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[[Category: Michel G]]

Current revision

1,3-ALPHA-1,4-BETA-D-GALACTOSE-4-SULFATE- 3,6-ANHYDRO-D-GALACTOSE-2-SULFATE 4 GALACTOHYDROLASE

PDB ID 1h80

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