1h8e

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (12:20, 13 December 2023) (edit) (undo)
 
(23 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1h8e.gif|left|200px]]<br />
 
-
<applet load="1h8e" size="450" color="white" frame="true" align="right" spinBox="true"
 
-
caption="1h8e, resolution 2.0&Aring;" />
 
-
'''(ADP.ALF4)2(ADP.SO4) BOVINE F1-ATPASE (ALL THREE CATALYTIC SITES OCCUPIED)'''<br />
 
-
==Overview==
+
==(ADP.AlF4)2(ADP.SO4) bovine F1-ATPase (all three catalytic sites occupied)==
-
The crystal structure of a novel aluminium fluoride inhibited form of, bovine mitochondrial F(1)-ATPase has been determined at 2 A resolution. In, contrast to all previously determined structures of the bovine enzyme, all, three catalytic sites are occupied by nucleotide. The subunit that did not, bind nucleotide in previous structures binds ADP and sulfate (mimicking, phosphate), and adopts a "half-closed" conformation. This structure, probably represents the posthydrolysis, pre-product release step on the, catalytic pathway. A catalytic scheme for hydrolysis (and synthesis) at, physiological rates and a mechanism for the ATP-driven rotation of the, gamma subunit are proposed based on the crystal structures of the bovine, enzyme.
+
<StructureSection load='1h8e' size='340' side='right'caption='[[1h8e]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[1h8e]] is a 9 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1H8E OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1H8E FirstGlance]. <br>
 +
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
 +
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=ALF:TETRAFLUOROALUMINATE+ION'>ALF</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1h8e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1h8e OCA], [https://pdbe.org/1h8e PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1h8e RCSB], [https://www.ebi.ac.uk/pdbsum/1h8e PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1h8e ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/ATPA_BOVIN ATPA_BOVIN] Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F(1). Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits. Subunit alpha does not bear the catalytic high-affinity ATP-binding sites (By similarity).
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/h8/1h8e_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1h8e ConSurf].
 +
<div style="clear:both"></div>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
The crystal structure of a novel aluminium fluoride inhibited form of bovine mitochondrial F(1)-ATPase has been determined at 2 A resolution. In contrast to all previously determined structures of the bovine enzyme, all three catalytic sites are occupied by nucleotide. The subunit that did not bind nucleotide in previous structures binds ADP and sulfate (mimicking phosphate), and adopts a "half-closed" conformation. This structure probably represents the posthydrolysis, pre-product release step on the catalytic pathway. A catalytic scheme for hydrolysis (and synthesis) at physiological rates and a mechanism for the ATP-driven rotation of the gamma subunit are proposed based on the crystal structures of the bovine enzyme.
-
==About this Structure==
+
Structure of bovine mitochondrial F(1)-ATPase with nucleotide bound to all three catalytic sites: implications for the mechanism of rotary catalysis.,Menz RI, Walker JE, Leslie AG Cell. 2001 Aug 10;106(3):331-41. PMID:11509182<ref>PMID:11509182</ref>
-
1H8E is a [[http://en.wikipedia.org/wiki/Protein_complex Protein complex]] structure of sequences from [[http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]] with MG, ALF, SO4, ADP and GOL as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/Transferred_entry:_3.6.3.14 Transferred entry: 3.6.3.14]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.1.34 3.6.1.34]]. Structure known Active Sites: AC1, AC2, AC3, AC4, AC5, AC6, AC7, AC8, AC9, BC1, BC2, BC3, BC4, BC5, BC6, CAT and PLP. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1H8E OCA]].
+
-
==Reference==
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
Structure of bovine mitochondrial F(1)-ATPase with nucleotide bound to all three catalytic sites: implications for the mechanism of rotary catalysis., Menz RI, Walker JE, Leslie AG, Cell. 2001 Aug 10;106(3):331-41. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11509182 11509182]
+
</div>
-
[[Category: Bos taurus]]
+
<div class="pdbe-citations 1h8e" style="background-color:#fffaf0;"></div>
-
[[Category: Protein complex]]
+
-
[[Category: Transferred entry: 3.6.3.14]]
+
-
[[Category: Leslie, A.G.W.]]
+
-
[[Category: Menz, R.I.]]
+
-
[[Category: Walker, J.E.]]
+
-
[[Category: ADP]]
+
-
[[Category: ALF]]
+
-
[[Category: GOL]]
+
-
[[Category: MG]]
+
-
[[Category: SO4]]
+
-
[[Category: atp phosphorylase]]
+
-
[[Category: atp phosphorylase (h+ transporting)]]
+
-
[[Category: atp synthase]]
+
-
[[Category: f1-atpase]]
+
-
[[Category: f1fo atp synthase]]
+
-
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 15:32:06 2007''
+
==See Also==
 +
*[[ATPase 3D structures|ATPase 3D structures]]
 +
== References ==
 +
<references/>
 +
__TOC__
 +
</StructureSection>
 +
[[Category: Bos taurus]]
 +
[[Category: Large Structures]]
 +
[[Category: Leslie AGW]]
 +
[[Category: Menz RI]]
 +
[[Category: Walker JE]]

Current revision

(ADP.AlF4)2(ADP.SO4) bovine F1-ATPase (all three catalytic sites occupied)

PDB ID 1h8e

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1h8e"
Personal tools