1yg2

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[[Image:1yg2.gif|left|200px]]<br /><applet load="1yg2" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1yg2, resolution 2.20&Aring;" />
 
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'''Structure of the Vibrio cholerae virulence activator AphA'''<br />
 
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==Overview==
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==Structure of the Vibrio cholerae virulence activator AphA==
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AphA is a member of a new and largely uncharacterized family of transcriptional activators that is required for initiating virulence gene expression in Vibrio cholerae, the causative agent of the frequently fatal epidemic diarrheal disease cholera. AphA activates transcription by an unusual mechanism that appears to involve a direct interaction with the LysR-type regulator AphB at the tcpPH promoter. As a first step toward understanding the molecular basis for tcpPH activation by AphA and AphB, we have determined the crystal structure of AphA to 2.2 angstrom resolution. AphA is a dimer with an N-terminal winged helix DNA binding domain that is architecturally similar to that of the MarR family of transcriptional regulators. Unlike this family, however, AphA has a unique C-terminal antiparallel coiled coil domain that serves as its primary dimerization interface. AphA monomers are highly unstable by themselves and form a linked topology, requiring the protein to partially unfold to form the dimer. The structure of AphA also provides insights into how it cooperates with AphB to activate transcription, most likely by forming a heterotetrameric complex at the tcpPH promoter.
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<StructureSection load='1yg2' size='340' side='right'caption='[[1yg2]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1yg2]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Vibrio_cholerae Vibrio cholerae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YG2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1YG2 FirstGlance]. <br>
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1YG2 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Vibrio_cholerae Vibrio cholerae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YG2 OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1yg2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1yg2 OCA], [https://pdbe.org/1yg2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1yg2 RCSB], [https://www.ebi.ac.uk/pdbsum/1yg2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1yg2 ProSAT]</span></td></tr>
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==Reference==
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</table>
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Crystal structure of the virulence gene activator AphA from Vibrio cholerae reveals it is a novel member of the winged helix transcription factor superfamily., De Silva RS, Kovacikova G, Lin W, Taylor RK, Skorupski K, Kull FJ, J Biol Chem. 2005 Apr 8;280(14):13779-83. Epub 2005 Jan 12. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15647287 15647287]
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== Function ==
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[[Category: Single protein]]
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[https://www.uniprot.org/uniprot/Q9X399_VIBCL Q9X399_VIBCL]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/yg/1yg2_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1yg2 ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Vibrio cholerae]]
[[Category: Vibrio cholerae]]
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[[Category: Kovacikova, G.]]
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[[Category: De Silva RS]]
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[[Category: Kull, F J.]]
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[[Category: Kovacikova G]]
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[[Category: Lin, W.]]
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[[Category: Kull FJ]]
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[[Category: Silva, R S.De.]]
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[[Category: Lin W]]
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[[Category: Skorupski, K.]]
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[[Category: Skorupski K]]
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[[Category: Taylor, R T.]]
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[[Category: Taylor RT]]
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[[Category: virulence factor; vibrio cholerae; winged helix; transcription factor]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:05:00 2008''
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Current revision

Structure of the Vibrio cholerae virulence activator AphA

PDB ID 1yg2

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