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4ag0

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[[Image:4ag0.jpg|left|200px]]
 
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{{STRUCTURE_4ag0| PDB=4ag0 | SCENE= }}
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==Crystal structure of FimX EAL domain==
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<StructureSection load='4ag0' size='340' side='right'caption='[[4ag0]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[4ag0]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4AG0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4AG0 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.304&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ag0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ag0 OCA], [https://pdbe.org/4ag0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ag0 RCSB], [https://www.ebi.ac.uk/pdbsum/4ag0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ag0 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q9HUK6_PSEAE Q9HUK6_PSEAE]
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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FimX is a large multidomain protein containing an EAL domain and involved in twitching motility in Pseudomonas aeruginosa. We present here two crystallographic structures of the EAL domain of FimX (residues 438-686): one of the apo form and the other of a complex with 5'-pGpG, the reaction product of the hydrolysis of c-di-GMP. In both crystal forms, the EAL domains form a dimer delimiting a large cavity encompassing the catalytic pockets. The ligand is trapped in this cavity by its sugar phosphate moiety. We confirmed by NMR that the guanine bases are not involved in the interaction in solution. We solved here the first structure of an EAL domain bound to the reaction product 5'-pGpG. Though isolated FimX EAL domain has a very low catalytic activity, which would not be significant compared to other catalytic EAL domains, the structure with the product of the reaction can provides some hints in the mechanism of hydrolysis of the c-di-GMP by EAL domains.
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===Crystal structure of FimX EAL domain===
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Crystal Structure of an EAL Domain in Complex with Reaction Product 5'-pGpG.,Robert-Paganin J, Nonin-Lecomte S, Rety S PLoS One. 2012;7(12):e52424. doi: 10.1371/journal.pone.0052424. Epub 2012 Dec 20. PMID:23285035<ref>PMID:23285035</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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==About this Structure==
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</div>
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[[4ag0]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4AG0 OCA].
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<div class="pdbe-citations 4ag0" style="background-color:#fffaf0;"></div>
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[[Category: Cyclic-guanylate-specific phosphodiesterase]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Pseudomonas aeruginosa]]
[[Category: Pseudomonas aeruginosa]]
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[[Category: Nonin-Lecomte, S.]]
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[[Category: Nonin-Lecomte S]]
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[[Category: Rety, S.]]
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[[Category: Rety S]]
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[[Category: Robert-Paganin, J.]]
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[[Category: Robert-Paganin J]]
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[[Category: Biofilm]]
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[[Category: C-digmp]]
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[[Category: Hydrolase]]
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[[Category: Phosphodiesterase]]
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Current revision

Crystal structure of FimX EAL domain

PDB ID 4ag0

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