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| - | [[Image:1yh0.gif|left|200px]]<br /><applet load="1yh0" size="350" color="white" frame="true" align="right" spinBox="true" | + | #REDIRECT [[3kzc]] This PDB entry is obsolete and replaced by 3kzc |
| - | caption="1yh0, resolution 2.20Å" />
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| - | '''Crystal Structure of Acetylornithine Transcarbamylase'''<br />
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| - | ==Overview==
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| - | We have identified in Xanthomonas campestris a novel N-acetylornithine transcarbamylase that replaces ornithine transcarbamylase in the canonic arginine biosynthetic pathway of several Eubacteria. The crystal structures of the protein in the presence and absence of the reaction product, N-acetylcitrulline, were determined. This new family of transcarbamylases lacks the DxxSMG motif that is characteristic of all ornithine transcarbamylases (OTCases) and contains a novel proline-rich loop that forms part of the active site. The specificity for N-acetylornithine is conferred by hydrogen bonding with residues in the proline-rich loop via water molecules and by hydrophobic interactions with residues from the adjacent 80's, 120's, and proline-rich loops. This novel protein structure provides a starting point for rational design of specific analogs that may be useful in combating human and plant pathogens that utilize acetylornithine transcarbamylase rather than ornithine transcarbamylase.
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| - | ==About this Structure==
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| - | 1YH0 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Xanthomonas_campestris Xanthomonas campestris] with <scene name='pdbligand=SO4:'>SO4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YH0 OCA].
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| - | ==Reference==
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| - | Crystal structure of N-acetylornithine transcarbamylase from Xanthomonas campestris: a novel enzyme in a new arginine biosynthetic pathway found in several eubacteria., Shi D, Morizono H, Yu X, Roth L, Caldovic L, Allewell NM, Malamy MH, Tuchman M, J Biol Chem. 2005 Apr 15;280(15):14366-9. Epub 2005 Feb 24. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15731101 15731101]
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| - | [[Category: Single protein]]
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| - | [[Category: Xanthomonas campestris]]
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| - | [[Category: Allewell, N M.]]
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| - | [[Category: Caldovic, L.]]
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| - | [[Category: Malamy, M H.]]
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| - | [[Category: Morizono, H.]]
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| - | [[Category: Roth, L.]]
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| - | [[Category: Shi, D.]]
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| - | [[Category: Tuchman, M.]]
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| - | [[Category: Yu, X.]]
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| - | [[Category: SO4]]
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| - | [[Category: acetylcitrulline]]
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| - | [[Category: acetylornithine]]
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| - | [[Category: argf]]
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| - | [[Category: transcarbamylase]]
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| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:05:13 2008''
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