3ef4

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[[Image:3ef4.png|left|200px]]
 
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{{STRUCTURE_3ef4| PDB=3ef4 | SCENE= }}
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==Crystal structure of native pseudoazurin from Hyphomicrobium denitrificans==
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<StructureSection load='3ef4' size='340' side='right'caption='[[3ef4]], [[Resolution|resolution]] 1.18&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3ef4]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Hyphomicrobium_denitrificans Hyphomicrobium denitrificans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EF4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3EF4 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.18&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ef4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ef4 OCA], [https://pdbe.org/3ef4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ef4 RCSB], [https://www.ebi.ac.uk/pdbsum/3ef4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ef4 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/A7VL37_9HYPH A7VL37_9HYPH]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ef/3ef4_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3ef4 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The crystal structure of native pseudoazurin (HdPAz) from the methylotrophic denitrifying bacterium Hyphomicrobium denitrificans has been determined at a resolution of 1.18 A. After refinement with SHELX employing anisotropic displacement parameters and riding H atoms, R(work) and R(free) were 0.135 and 0.169, respectively. Visualization of the anisotropic displacement parameters as thermal ellipsoids provided insight into the atomic motion within the perturbed type 1 Cu site. The asymmetric unit includes three HdPAz molecules which are tightly packed by head-to-head cupredoxin dimer formation. The shape of the Cu-atom ellipsoid implies significant vibrational motion diagonal to the equatorial xy plane defined by the three ligands (two His and one Cys). The geometric parameters of the type 1 Cu site in the HdPAz structure differ unambiguously from those of other pseudoazurins. It is demonstrated that their structural aspects are consistent with the unique visible absorption spectrum.
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===Crystal structure of native pseudoazurin from Hyphomicrobium denitrificans===
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Atomic resolution structure of pseudoazurin from the methylotrophic denitrifying bacterium Hyphomicrobium denitrificans: structural insights into its spectroscopic properties.,Hira D, Nojiri M, Suzuki S Acta Crystallogr D Biol Crystallogr. 2009 Jan;65(Pt 1):85-92. Epub 2008, Dec 18. PMID:19153470<ref>PMID:19153470</ref>
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{{ABSTRACT_PUBMED_19153470}}
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3ef4" style="background-color:#fffaf0;"></div>
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==About this Structure==
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==See Also==
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[[3ef4]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Hyphomicrobium_denitrificans Hyphomicrobium denitrificans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EF4 OCA].
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*[[Pseudoazurin|Pseudoazurin]]
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:019153470</ref><references group="xtra"/>
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__TOC__
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</StructureSection>
[[Category: Hyphomicrobium denitrificans]]
[[Category: Hyphomicrobium denitrificans]]
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[[Category: Hira, D.]]
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[[Category: Large Structures]]
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[[Category: Nojiri, M.]]
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[[Category: Hira D]]
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[[Category: Suzuki, S.]]
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[[Category: Nojiri M]]
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[[Category: Blue copper protein]]
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[[Category: Suzuki S]]
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[[Category: Electron transfer]]
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[[Category: Electron transport]]
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Current revision

Crystal structure of native pseudoazurin from Hyphomicrobium denitrificans

PDB ID 3ef4

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