1yj7

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[[Image:1yj7.gif|left|200px]]<br /><applet load="1yj7" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1yj7, resolution 1.8&Aring;" />
 
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'''Crystal structure of enteropathogenic E.coli (EPEC) type III secretion system protein EscJ'''<br />
 
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==Overview==
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==Crystal structure of enteropathogenic E.coli (EPEC) type III secretion system protein EscJ==
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Type III secretion systems (TTSSs) are multi-protein macromolecular 'machines' that have a central function in the virulence of many Gram-negative pathogens by directly mediating the secretion and translocation of bacterial proteins (termed effectors) into the cytoplasm of eukaryotic cells. Most of the 20 unique structural components constituting this secretion apparatus are highly conserved among animal and plant pathogens and are also evolutionarily related to proteins in the flagellar-specific export system. Recent electron microscopy experiments have revealed the gross 'needle-shaped' morphology of the TTSS, yet a detailed understanding of the structural characteristics and organization of these protein components within the bacterial membranes is lacking. Here we report the 1.8-A crystal structure of EscJ from enteropathogenic Escherichia coli (EPEC), a member of the YscJ/PrgK family whose oligomerization represents one of the earliest events in TTSS assembly. Crystal packing analysis and molecular modelling indicate that EscJ could form a large 24-subunit 'ring' superstructure with extensive grooves, ridges and electrostatic features. Electron microscopy, labelling and mass spectrometry studies on the orthologous Salmonella typhimurium PrgK within the context of the assembled TTSS support the stoichiometry, membrane association and surface accessibility of the modelled ring. We propose that the YscJ/PrgK protein family functions as an essential molecular platform for TTSS assembly.
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<StructureSection load='1yj7' size='340' side='right'caption='[[1yj7]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1yj7]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YJ7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1YJ7 FirstGlance]. <br>
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1YJ7 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=PO4:'>PO4</scene> and <scene name='pdbligand=GOL:'>GOL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YJ7 OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1yj7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1yj7 OCA], [https://pdbe.org/1yj7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1yj7 RCSB], [https://www.ebi.ac.uk/pdbsum/1yj7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1yj7 ProSAT]</span></td></tr>
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Structural characterization of the molecular platform for type III secretion system assembly., Yip CK, Kimbrough TG, Felise HB, Vuckovic M, Thomas NA, Pfuetzner RA, Frey EA, Finlay BB, Miller SI, Strynadka NC, Nature. 2005 Jun 2;435(7042):702-7. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15931226 15931226]
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q8VQD3_ECOLX Q8VQD3_ECOLX]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/yj/1yj7_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1yj7 ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Felise, H B.]]
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[[Category: Felise HB]]
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[[Category: Finlay, B B.]]
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[[Category: Finlay BB]]
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[[Category: Frey, E A.]]
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[[Category: Frey EA]]
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[[Category: Kimbrough, T G.]]
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[[Category: Kimbrough TG]]
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[[Category: Miller, S I.]]
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[[Category: Miller SI]]
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[[Category: Pfuetzner, R A.]]
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[[Category: Pfuetzner RA]]
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[[Category: Strynadka, N C.J.]]
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[[Category: Strynadka NCJ]]
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[[Category: Thomas, N A.]]
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[[Category: Thomas NA]]
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[[Category: Vuckovic, M.]]
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[[Category: Vuckovic M]]
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[[Category: Yip, C K.]]
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[[Category: Yip CK]]
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[[Category: GOL]]
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[[Category: PO4]]
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[[Category: extended linker]]
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[[Category: mixed alpha/beta]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:05:51 2008''
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Current revision

Crystal structure of enteropathogenic E.coli (EPEC) type III secretion system protein EscJ

PDB ID 1yj7

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