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3e4b
From Proteopedia
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| - | [[Image:3e4b.png|left|200px]] | ||
| - | + | ==Crystal structure of AlgK from Pseudomonas fluorescens WCS374r== | |
| + | <StructureSection load='3e4b' size='340' side='right'caption='[[3e4b]], [[Resolution|resolution]] 2.50Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[3e4b]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_fluorescens_liquefaciens"_flugge_1886 "bacillus fluorescens liquefaciens" flugge 1886]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3E4B OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3E4B FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> | ||
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">algK ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=294 "Bacillus fluorescens liquefaciens" Flugge 1886])</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3e4b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3e4b OCA], [https://pdbe.org/3e4b PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3e4b RCSB], [https://www.ebi.ac.uk/pdbsum/3e4b PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3e4b ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Evolutionary Conservation == | ||
| + | [[Image:Consurf_key_small.gif|200px|right]] | ||
| + | Check<jmol> | ||
| + | <jmolCheckbox> | ||
| + | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/e4/3e4b_consurf.spt"</scriptWhenChecked> | ||
| + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | ||
| + | <text>to colour the structure by Evolutionary Conservation</text> | ||
| + | </jmolCheckbox> | ||
| + | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3e4b ConSurf]. | ||
| + | <div style="clear:both"></div> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The opportunistic pathogen Pseudomonas aeruginosa causes chronic biofilm infections in cystic fibrosis patients. During colonization of the lung, P. aeruginosa converts to a mucoid phenotype characterized by overproduction of the exopolysaccharide alginate. Here we show that AlgK, a protein essential for production of high molecular weight alginate, is an outer membrane lipoprotein that contributes to the correct localization of the porin AlgE. Our 2.5 A structure shows AlgK is composed of 9.5 tetratricopeptide-like repeats, and three putative sites of protein-protein interaction have been identified. Bioinformatics analysis suggests that BcsA, PgaA, and PelB, involved in the production and export of cellulose, poly-beta-1,6-N-Acetyl-D-glucosamine, and Pel exopolysaccharide, respectively, share the same topology as AlgK/E. Together, our data suggest that AlgK plays a role in the assembly of the alginate biosynthetic complex and represents the periplasmic component of a new type of outer membrane secretin that differs from canonical bacterial capsular polysaccharide secretion systems. | ||
| - | + | AlgK is a TPR-containing protein and the periplasmic component of a novel exopolysaccharide secretin.,Keiski CL, Harwich M, Jain S, Neculai AM, Yip P, Robinson H, Whitney JC, Riley L, Burrows LL, Ohman DE, Howell PL Structure. 2010 Feb 10;18(2):265-73. PMID:20159471<ref>PMID:20159471</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | <div class="pdbe-citations 3e4b" style="background-color:#fffaf0;"></div> | |
| - | + | == References == | |
| - | + | <references/> | |
| - | == | + | __TOC__ |
| - | < | + | </StructureSection> |
| - | [[Category: | + | [[Category: Bacillus fluorescens liquefaciens flugge 1886]] |
| - | [[Category: Burrows, L L | + | [[Category: Large Structures]] |
| - | [[Category: Harwich, M | + | [[Category: Burrows, L L]] |
| - | [[Category: Howell, P L | + | [[Category: Harwich, M]] |
| - | [[Category: Jain, S | + | [[Category: Howell, P L]] |
| - | [[Category: Keiski, C L | + | [[Category: Jain, S]] |
| - | [[Category: Neculai, A M | + | [[Category: Keiski, C L]] |
| - | [[Category: Ohman, D E | + | [[Category: Neculai, A M]] |
| - | [[Category: Robinson, H | + | [[Category: Ohman, D E]] |
| - | [[Category: Whitney, J C | + | [[Category: Robinson, H]] |
| - | [[Category: Yip, P | + | [[Category: Whitney, J C]] |
| + | [[Category: Yip, P]] | ||
[[Category: Alginate biosynthesis]] | [[Category: Alginate biosynthesis]] | ||
[[Category: Algk]] | [[Category: Algk]] | ||
Current revision
Crystal structure of AlgK from Pseudomonas fluorescens WCS374r
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Categories: Bacillus fluorescens liquefaciens flugge 1886 | Large Structures | Burrows, L L | Harwich, M | Howell, P L | Jain, S | Keiski, C L | Neculai, A M | Ohman, D E | Robinson, H | Whitney, J C | Yip, P | Alginate biosynthesis | Algk | Protein binding | Pseudomona | Superhelix | Tetratricopeptide repeat
