3e1k

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[[Image:3e1k.png|left|200px]]
 
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{{STRUCTURE_3e1k| PDB=3e1k | SCENE= }}
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==Crystal structure of Kluyveromyces lactis Gal80p in complex with the acidic activation domain of Gal4p==
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<StructureSection load='3e1k' size='340' side='right'caption='[[3e1k]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3e1k]] is a 16 chain structure with sequence from [https://en.wikipedia.org/wiki/Kluyveromyces_lactis Kluyveromyces lactis] and [https://en.wikipedia.org/wiki/Kluyveromyces_lactis_NRRL_Y-1140 Kluyveromyces lactis NRRL Y-1140]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3E1K OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3E1K FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3e1k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3e1k OCA], [https://pdbe.org/3e1k PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3e1k RCSB], [https://www.ebi.ac.uk/pdbsum/3e1k PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3e1k ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/GAL80_KLULA GAL80_KLULA] This protein is a negative regulator for the gene expression of the lactose/galactose metabolic genes. It seems to block activation by the transcriptional activator LAC9 in the absence of an inducing sugar.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/e1/3e1k_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3e1k ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The GAL genes, which encode the enzymes required for normal galactose metabolism in yeast, are transcriptionally regulated by three proteins: Gal4p, an activator; Gal80p, an inhibitor; and Gal3p, a galactose sensor. These proteins control the switch between inert and active gene expression. The transcriptional activation function of Gal4p is rendered inactive in the presence of Gal80p. Here we present the three-dimensional structure of a complex between the acidic activation domain of Gal4p and Gal80p. The transactivation domain initiates with an extended region of polypeptide chain followed by two turns of an amphipathic alpha-helix. It fits into and across a deep cleft within the Gal80p dimer with the protein-protein interface defined primarily by hydrophobic interactions. A disordered loop in the apo-Gal80p structure (Asp-309 to Ser-316) becomes well-defined upon binding of the transactivation domain. This investigation provides a new molecular scaffold for understanding previous biochemical and genetic studies.
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===Crystal structure of Kluyveromyces lactis Gal80p in complex with the acidic activation domain of Gal4p===
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The interaction between an acidic transcriptional activator and its inhibitor. The molecular basis of Gal4p recognition by Gal80p.,Thoden JB, Ryan LA, Reece RJ, Holden HM J Biol Chem. 2008 Oct 31;283(44):30266-72. Epub 2008 Aug 13. PMID:18701455<ref>PMID:18701455</ref>
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{{ABSTRACT_PUBMED_18701455}}
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3e1k" style="background-color:#fffaf0;"></div>
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==About this Structure==
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==See Also==
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[[3e1k]] is a 16 chain structure with sequence from [http://en.wikipedia.org/wiki/Kluyveromyces_lactis Kluyveromyces lactis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3E1K OCA].
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*[[Gal3-Gal80-Gal4|Gal3-Gal80-Gal4]]
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:018701455</ref><references group="xtra"/>
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__TOC__
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</StructureSection>
[[Category: Kluyveromyces lactis]]
[[Category: Kluyveromyces lactis]]
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[[Category: Holden, H M.]]
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[[Category: Kluyveromyces lactis NRRL Y-1140]]
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[[Category: Thoden, J B.]]
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[[Category: Large Structures]]
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[[Category: Activator]]
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[[Category: Holden HM]]
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[[Category: Carbohydrate metabolism]]
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[[Category: Thoden JB]]
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[[Category: Dna-binding]]
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[[Category: Galactose metabolism]]
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[[Category: Metal-binding]]
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[[Category: Nucleus]]
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[[Category: Repressor]]
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[[Category: Trans-activation]]
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[[Category: Transcription]]
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[[Category: Transcription regulation]]
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[[Category: Transctiption]]
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Current revision

Crystal structure of Kluyveromyces lactis Gal80p in complex with the acidic activation domain of Gal4p

PDB ID 3e1k

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