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3e9s

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[[Image:3e9s.png|left|200px]]
 
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{{STRUCTURE_3e9s| PDB=3e9s | SCENE= }}
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==A new class of papain-like protease/deubiquitinase inhibitors blocks SARS virus replication==
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<StructureSection load='3e9s' size='340' side='right'caption='[[3e9s]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3e9s]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Cvhsa Cvhsa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3E9S OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3E9S FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=TTT:5-AMINO-2-METHYL-N-[(1R)-1-NAPHTHALEN-1-YLETHYL]BENZAMIDE'>TTT</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=OCS:CYSTEINESULFONIC+ACID'>OCS</scene></td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2fe8|2fe8]]</div></td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">1a ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=227859 CVHSA])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3e9s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3e9s OCA], [https://pdbe.org/3e9s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3e9s RCSB], [https://www.ebi.ac.uk/pdbsum/3e9s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3e9s ProSAT]</span></td></tr>
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</table>
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== Function ==
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[[https://www.uniprot.org/uniprot/R1A_CVHSA R1A_CVHSA]] The papain-like proteinase (PL-PRO) is responsible for the cleavages located at the N-terminus of replicase polyprotein. In addition, PL-PRO possesses a deubiquitinating/deISGylating activity and processes both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains from cellular substrates. Antagonizes innate immune induction of type I interferon by blocking the phosphorylation, dimerization and subsequent nuclear translocation of host IRF-3.<ref>PMID:17024178</ref> <ref>PMID:17692280</ref> <ref>PMID:19369340</ref> The main proteinase 3CL-PRO is responsible for the majority of cleavages as it cleaves the C-terminus of replicase polyprotein at 11 sites. Recognizes substrates containing the core sequence [ILMVF]-Q-|-[SGACN]. Inhibited by the substrate-analog Cbz-Val-Asn-Ser-Thr-Leu-Gln-CMK (By similarity). Also contains an ADP-ribose-1''-phosphate (ADRP)-binding function.<ref>PMID:17024178</ref> <ref>PMID:17692280</ref> <ref>PMID:19369340</ref> Nsp7-nsp8 hexadecamer may possibly confer processivity to the polymerase, maybe by binding to dsRNA or by producing primers utilized by the latter.<ref>PMID:17024178</ref> <ref>PMID:17692280</ref> <ref>PMID:19369340</ref> Nsp9 is a ssRNA-binding protein.<ref>PMID:17024178</ref> <ref>PMID:17692280</ref> <ref>PMID:19369340</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/e9/3e9s_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3e9s ConSurf].
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<div style="clear:both"></div>
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===A new class of papain-like protease/deubiquitinase inhibitors blocks SARS virus replication===
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==See Also==
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*[[Virus protease 3D structures|Virus protease 3D structures]]
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{{ABSTRACT_PUBMED_018852458}}
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== References ==
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<references/>
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==About this Structure==
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__TOC__
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[[3e9s]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Sars_coronavirus Sars coronavirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3E9S OCA].
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</StructureSection>
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[[Category: Sars coronavirus]]
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[[Category: Cvhsa]]
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[[Category: Mesecar, A D.]]
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[[Category: Large Structures]]
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[[Category: Pegan, S.]]
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[[Category: Mesecar, A D]]
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[[Category: Ratia, K.]]
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[[Category: Pegan, S]]
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[[Category: Ratia, K]]
[[Category: Coronavirus]]
[[Category: Coronavirus]]
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[[Category: Cytoplasm]]
[[Category: Hydrolase]]
[[Category: Hydrolase]]
[[Category: Membrane]]
[[Category: Membrane]]
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[[Category: Plpro]]
[[Category: Plpro]]
[[Category: Protease]]
[[Category: Protease]]
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[[Category: Ribosomal frameshifting]]
[[Category: Rna-binding]]
[[Category: Rna-binding]]
[[Category: Sar]]
[[Category: Sar]]
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[[Category: Transmembrane]]
[[Category: Transmembrane]]
[[Category: Virus]]
[[Category: Virus]]
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[[Category: Zinc]]
[[Category: Zinc-finger]]
[[Category: Zinc-finger]]

Current revision

A new class of papain-like protease/deubiquitinase inhibitors blocks SARS virus replication

PDB ID 3e9s

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OCA

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