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3dw4

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[[Image:3dw4.png|left|200px]]
 
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{{STRUCTURE_3dw4| PDB=3dw4 | SCENE= }}
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==Crystal Structure of the Sarcin/Ricin Domain from E. COLI 23 S rRNA, U2650-OCH3 modified==
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<StructureSection load='3dw4' size='340' side='right'caption='[[3dw4]], [[Resolution|resolution]] 0.97&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3dw4]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3DW4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3DW4 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 0.97&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=OMU:O2-METHYLURIDINE+5-MONOPHOSPHATE'>OMU</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3dw4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3dw4 OCA], [https://pdbe.org/3dw4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3dw4 RCSB], [https://www.ebi.ac.uk/pdbsum/3dw4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3dw4 ProSAT]</span></td></tr>
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</table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Site-specific 2'-methylseleno RNA labeling is a promising tool for tackling the phase problem in RNA crystallography. We have developed an efficient strategy for crystallization and structure determination of RNA and RNA/protein complexes based on preliminary crystallization screening of 2'-OCH(3)-modified RNA sequences, prior to the replacement of 2'-OCH(3) groups with their 2'-SeCH(3) counterparts. The method exploits the similar crystallization properties of 2'-OCH(3)- and 2'-SeCH(3)-modified RNAs and has been successfully validated for two test cases. In addition, our data show that 2'-SeCH(3)-modified RNA have an increased resistance to X-ray radiolysis in comparison with commonly used 5-halogen-modified RNA, which permits collection of experimental electron density maps of remarkable quality.
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===Crystal Structure of the Sarcin/Ricin Domain from E. COLI 23 S rRNA, U2650-OCH3 modified===
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A fast selenium derivatization strategy for crystallization and phasing of RNA structures.,Olieric V, Rieder U, Lang K, Serganov A, Schulze-Briese C, Micura R, Dumas P, Ennifar E RNA. 2009 Apr;15(4):707-15. Epub 2009 Feb 18. PMID:19228585<ref>PMID:19228585</ref>
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{{ABSTRACT_PUBMED_19228585}}
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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==About this Structure==
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<div class="pdbe-citations 3dw4" style="background-color:#fffaf0;"></div>
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[[3dw4]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3DW4 OCA].
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== References ==
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<references/>
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==Reference==
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__TOC__
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<ref group="xtra">PMID:019228585</ref><references group="xtra"/>
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</StructureSection>
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[[Category: Dumas, P.]]
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[[Category: Large Structures]]
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[[Category: Ennifar, E.]]
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[[Category: Dumas P]]
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[[Category: Lang, K.]]
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[[Category: Ennifar E]]
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[[Category: Micura, R.]]
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[[Category: Lang K]]
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[[Category: Olieric, V.]]
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[[Category: Micura R]]
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[[Category: Rieder, U.]]
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[[Category: Olieric V]]
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[[Category: Schulze-Briese, C.]]
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[[Category: Rieder U]]
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[[Category: Serganov, A.]]
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[[Category: Schulze-Briese C]]
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[[Category: Rna]]
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[[Category: Serganov A]]
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[[Category: Sarcin ricin loop]]
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Current revision

Crystal Structure of the Sarcin/Ricin Domain from E. COLI 23 S rRNA, U2650-OCH3 modified

PDB ID 3dw4

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