2llz

From Proteopedia

(Difference between revisions)

Current revision

GhoS (YjdK) monomer

Structural highlights

2llz is a 1 chain structure with sequence from Escherichia coli K-12. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	Solution NMR
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

GHOS_ECOLI Antitoxin component of a type V toxin-antitoxin (TA) system. Neutralizes the toxic effects of toxin GhoT by digesting ghoT transcripts in a sequence-specific manner (PubMed:22941047). In concert with GhoT is involved in reducing cell growth during antibacterial stress (PubMed:24373067). Overexpression leads to transcript level reduction of 20 other mRNAs involved in purine or pyrimidine synthesis and transport. Not seen to bind its own promoter DNA (PubMed:22941047).^[1] ^[2]

Publication Abstract from PubMed

Among bacterial toxin-antitoxin systems, to date no antitoxin has been identified that functions by cleaving toxin mRNA. Here we show that YjdO (renamed GhoT) is a membrane lytic peptide that causes ghost cell formation (lysed cells with damaged membranes) and increases persistence (persister cells are tolerant to antibiotics without undergoing genetic change). GhoT is part of a new toxin-antitoxin system with YjdK (renamed GhoS) because in vitro RNA degradation studies, quantitative real-time reverse-transcription PCR and whole-transcriptome studies revealed that GhoS masks GhoT toxicity by cleaving specifically yjdO (ghoT) mRNA. Alanine substitutions showed that Arg28 is important for GhoS activity, and RNA sequencing indicated that the GhoS cleavage site is rich in U and A. The NMR structure of GhoS indicates it is related to the CRISPR-associated-2 RNase, and GhoS is a monomer. Hence, GhoT-GhoS is to our knowledge the first type V toxin-antitoxin system where a protein antitoxin inhibits the toxin by cleaving specifically its mRNA.

A new type V toxin-antitoxin system where mRNA for toxin GhoT is cleaved by antitoxin GhoS.,Wang X, Lord DM, Cheng HY, Osbourne DO, Hong SH, Sanchez-Torres V, Quiroga C, Zheng K, Herrmann T, Peti W, Benedik MJ, Page R, Wood TK Nat Chem Biol. 2012 Sep 2. doi: 10.1038/nchembio.1062. PMID:22941047^[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Wang X, Lord DM, Cheng HY, Osbourne DO, Hong SH, Sanchez-Torres V, Quiroga C, Zheng K, Herrmann T, Peti W, Benedik MJ, Page R, Wood TK. A new type V toxin-antitoxin system where mRNA for toxin GhoT is cleaved by antitoxin GhoS. Nat Chem Biol. 2012 Sep 2. doi: 10.1038/nchembio.1062. PMID:22941047 doi:http://dx.doi.org/10.1038/nchembio.1062
↑ Cheng HY, Soo VW, Islam S, McAnulty MJ, Benedik MJ, Wood TK. Toxin GhoT of the GhoT/GhoS toxin/antitoxin system damages the cell membrane to reduce adenosine triphosphate and to reduce growth under stress. Environ Microbiol. 2014 Jun;16(6):1741-54. PMID:24373067 doi:10.1111/1462-2920.12373
↑ Wang X, Lord DM, Cheng HY, Osbourne DO, Hong SH, Sanchez-Torres V, Quiroga C, Zheng K, Herrmann T, Peti W, Benedik MJ, Page R, Wood TK. A new type V toxin-antitoxin system where mRNA for toxin GhoT is cleaved by antitoxin GhoS. Nat Chem Biol. 2012 Sep 2. doi: 10.1038/nchembio.1062. PMID:22941047 doi:http://dx.doi.org/10.1038/nchembio.1062

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2llz"

Categories: Escherichia coli K-12 | Large Structures | Lord D | Page R | Peti W

@@ Line 1: / Line 1: @@
-[[Image:2llz.png|left|200px]]
-{{STRUCTURE_2llz|  PDB=2llz  |  SCENE=  }}
+==GhoS (YjdK) monomer==
+<StructureSection load='2llz' size='340' side='right'caption='[[2llz]]' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[2llz]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LLZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2LLZ FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2llz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2llz OCA], [https://pdbe.org/2llz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2llz RCSB], [https://www.ebi.ac.uk/pdbsum/2llz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2llz ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/GHOS_ECOLI GHOS_ECOLI] Antitoxin component of a type V toxin-antitoxin (TA) system. Neutralizes the toxic effects of toxin GhoT by digesting ghoT transcripts in a sequence-specific manner (PubMed:22941047). In concert with GhoT is involved in reducing cell growth during antibacterial stress (PubMed:24373067). Overexpression leads to transcript level reduction of 20 other mRNAs involved in purine or pyrimidine synthesis and transport. Not seen to bind its own promoter DNA (PubMed:22941047).<ref>PMID:22941047</ref> <ref>PMID:24373067</ref>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Among bacterial toxin-antitoxin systems, to date no antitoxin has been identified that functions by cleaving toxin mRNA. Here we show that YjdO (renamed GhoT) is a membrane lytic peptide that causes ghost cell formation (lysed cells with damaged membranes) and increases persistence (persister cells are tolerant to antibiotics without undergoing genetic change). GhoT is part of a new toxin-antitoxin system with YjdK (renamed GhoS) because in vitro RNA degradation studies, quantitative real-time reverse-transcription PCR and whole-transcriptome studies revealed that GhoS masks GhoT toxicity by cleaving specifically yjdO (ghoT) mRNA. Alanine substitutions showed that Arg28 is important for GhoS activity, and RNA sequencing indicated that the GhoS cleavage site is rich in U and A. The NMR structure of GhoS indicates it is related to the CRISPR-associated-2 RNase, and GhoS is a monomer. Hence, GhoT-GhoS is to our knowledge the first type V toxin-antitoxin system where a protein antitoxin inhibits the toxin by cleaving specifically its mRNA.
-===GhoS (YjdK) monomer===
+A new type V toxin-antitoxin system where mRNA for toxin GhoT is cleaved by antitoxin GhoS.,Wang X, Lord DM, Cheng HY, Osbourne DO, Hong SH, Sanchez-Torres V, Quiroga C, Zheng K, Herrmann T, Peti W, Benedik MJ, Page R, Wood TK Nat Chem Biol. 2012 Sep 2. doi: 10.1038/nchembio.1062. PMID:22941047<ref>PMID:22941047</ref>
-{{ABSTRACT_PUBMED_22941047}}
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
-==About this Structure==
+<div class="pdbe-citations 2llz" style="background-color:#fffaf0;"></div>
-[[2llz]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli_k-12 Escherichia coli k-12]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LLZ OCA].
+== References ==
-[[Category: Escherichia coli k-12]]
+<references/>
-[[Category: Lord, D.]]
+__TOC__
-[[Category: Page, R.]]
+</StructureSection>
-[[Category: Peti, W.]]
+[[Category: Escherichia coli K-12]]
-[[Category: Biofilm]]
+[[Category: Large Structures]]
-[[Category: Rnase]]
+[[Category: Lord D]]
-[[Category: Unknown function]]
+[[Category: Page R]]
+[[Category: Peti W]]

2llz

From Proteopedia

Current revision

GhoS (YjdK) monomer

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

Personal tools

Navigation

Search

Toolbox