1ux5

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[[Image:1ux5.png|left|200px]]
 
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{{STRUCTURE_1ux5| PDB=1ux5 | SCENE= }}
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==Crystal Structures of a Formin Homology-2 domain reveal a flexibly tethered dimer architecture==
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<StructureSection load='1ux5' size='340' side='right'caption='[[1ux5]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1ux5]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UX5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1UX5 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ux5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ux5 OCA], [https://pdbe.org/1ux5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ux5 RCSB], [https://www.ebi.ac.uk/pdbsum/1ux5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ux5 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/BNI1_YEAST BNI1_YEAST] Required for the assembly of F-actin structures, such as actin cables and stress fibers. Nucleates actin filaments. Binds to the barbed end of the actin filament and acts as leaky capper, slowing both polymerization and depolymerization. Protects the growing actin fiber from tight capping proteins and so increases the time of elongation and the total amount of F-actin. May organize microtubules by mediating spindle positioning and movement in the budding process. Potential target of the RHO family members.<ref>PMID:10085293</ref> <ref>PMID:14561409</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ux/1ux5_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ux5 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Formin proteins participate in a wide range of cytoskeletal processes in all eukaryotes. The defining feature of formins is a highly conserved approximately 400 residue region, the Formin Homology-2 (FH2) domain, which has recently been found to nucleate actin filaments. Here we report crystal structures of the S. cerevesiae Bni1p FH2 domain. The mostly alpha-helical FH2 domain forms a unique "tethered dimer" in which two elongated actin binding heads are tied together at either end by an unusual lasso and linker structure. Biochemical and crystallographic observations indicate that the dimer is stable but flexible, with flexibility between the two halves of the dimer conferred by the linker segments. Although each half of the dimer is competent to interact with filament ends, the intact dimer is required for actin nucleation and processive capping. The tethered dimer architecture may allow formins to stair-step on the barbed end of an elongating nascent filament.
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===CRYSTAL STRUCTURES OF A FORMIN HOMOLOGY-2 DOMAIN REVEAL A FLEXIBLY TETHERED DIMER ARCHITECTURE===
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Crystal structures of a Formin Homology-2 domain reveal a tethered dimer architecture.,Xu Y, Moseley JB, Sagot I, Poy F, Pellman D, Goode BL, Eck MJ Cell. 2004 Mar 5;116(5):711-23. PMID:15006353<ref>PMID:15006353</ref>
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{{ABSTRACT_PUBMED_15006353}}
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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==About this Structure==
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<div class="pdbe-citations 1ux5" style="background-color:#fffaf0;"></div>
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[[1ux5]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UX5 OCA].
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== References ==
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<references/>
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==Reference==
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__TOC__
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<ref group="xtra">PMID:015006353</ref><ref group="xtra">PMID:011740491</ref><references group="xtra"/>
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
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[[Category: Eck, M J.]]
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[[Category: Eck MJ]]
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[[Category: Goode, B L.]]
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[[Category: Goode BL]]
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[[Category: Moseley, J B.]]
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[[Category: Moseley JB]]
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[[Category: Pellman, D.]]
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[[Category: Pellman D]]
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[[Category: Poy, F.]]
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[[Category: Poy F]]
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[[Category: Sagot, I.]]
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[[Category: Sagot I]]
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[[Category: Xu, Y.]]
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[[Category: Xu Y]]
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[[Category: Fh2 actin cytoskeleton]]
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[[Category: Structural protein]]
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Current revision

Crystal Structures of a Formin Homology-2 domain reveal a flexibly tethered dimer architecture

PDB ID 1ux5

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