1ywf

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[[Image:1ywf.gif|left|200px]]<br /><applet load="1ywf" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1ywf, resolution 1.71&Aring;" />
 
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'''Crystal Structure of Mycobacterium Tuberculosis Protein Tyrosine Phosphatase PtpB'''<br />
 
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==Overview==
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==Crystal Structure of Mycobacterium Tuberculosis Protein Tyrosine Phosphatase PtpB==
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Intracellular pathogenic bacteria manipulate host signal transduction pathways to facilitate infection. Mycobacterium tuberculosis protein tyrosine phosphatases (PTPs) PtpA and PtpB are thought to be secreted into host cells and interfere with unidentified signals. To illuminate the mechanisms of regulation and substrate recognition, we determined the 1.7 A resolution crystal structure of PtpB in complex with the product phosphate. The protein adopts a simplified PTP fold, which combines features of the conventional PTPs and dual-specificity phosphatases. PtpB shows two unusual elaborations--a disordered, acidic loop and a flexible, two-helix lid that covers the active site--that are specific to mycobacterial orthologs. Biochemical studies suggest that substrate mimicry in the lid may protect the phosphatase from oxidative inactivation. The insertion and deletion of large structural elements in PtpB suggest that, outside the active site module, the PTP family is under unusual selective pressure that promotes changes in overall structure.
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<StructureSection load='1ywf' size='340' side='right'caption='[[1ywf]], [[Resolution|resolution]] 1.71&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1ywf]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YWF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1YWF FirstGlance]. <br>
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1YWF is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis] with <scene name='pdbligand=PO4:'>PO4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YWF OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.71&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ywf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ywf OCA], [https://pdbe.org/1ywf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ywf RCSB], [https://www.ebi.ac.uk/pdbsum/1ywf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ywf ProSAT], [https://www.topsan.org/Proteins/TBSGC/1ywf TOPSAN]</span></td></tr>
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Mycobacterium tuberculosis protein tyrosine phosphatase PtpB structure reveals a diverged fold and a buried active site., Grundner C, Ng HL, Alber T, Structure. 2005 Nov;13(11):1625-34. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16271885 16271885]
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/P96830_MYCTO P96830_MYCTO]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/yw/1ywf_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ywf ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Mycobacterium tuberculosis]]
[[Category: Mycobacterium tuberculosis]]
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[[Category: Single protein]]
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[[Category: Alber T]]
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[[Category: Alber, T.]]
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[[Category: Grundner C]]
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[[Category: Grundner, C.]]
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[[Category: Ng HL]]
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[[Category: Ng, H L.]]
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[[Category: TBSGC, TB Structural Genomics Consortium.]]
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[[Category: PO4]]
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[[Category: four stranded parallel beta sheet with flanking helices]]
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[[Category: protein structure initiative]]
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[[Category: psi]]
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[[Category: structural genomics]]
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[[Category: tb structural genomics consortium]]
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[[Category: tbsgc]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:09:46 2008''
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Current revision

Crystal Structure of Mycobacterium Tuberculosis Protein Tyrosine Phosphatase PtpB

PDB ID 1ywf

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