This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


1z0t

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (09:01, 14 February 2024) (edit) (undo)
 
(16 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1z0t.gif|left|200px]]<br /><applet load="1z0t" size="350" color="white" frame="true" align="right" spinBox="true"
 
-
caption="1z0t, resolution 3.00&Aring;" />
 
-
'''Crystal Structure of A. fulgidus Lon proteolytic domain'''<br />
 
-
==Overview==
+
==Crystal Structure of A. fulgidus Lon proteolytic domain==
-
Although macromolecular crystallography is rapidly becoming largely routine owing to advances in methods of data collection, structure solution and refinement, difficult cases are still common. To remind structural biologists about the kinds of crystallographic difficulties that might be encountered, case studies of several successfully completed structure determinations that utilized less than perfect crystals are discussed here. The structure of the proteolytic domain of Archaeoglobus fulgidus Lon was solved with crystals that contained superimposed orthorhombic and monoclinic lattices, a case not previously described for proteins. Another hexagonal crystal form of this protein exhibited an unusually high degree of non-isomorphism. Crystals of A. fulgidus Rio1 kinase exhibited both pseudosymmetry and twinning. Ways of identifying the observed phenomena and approaches to solving and refining macromolecular structures when only less than perfect crystals are available are discussed here.
+
<StructureSection load='1z0t' size='340' side='right'caption='[[1z0t]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
-
 
+
== Structural highlights ==
-
==About this Structure==
+
<table><tr><td colspan='2'>[[1z0t]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Archaeoglobus_fulgidus Archaeoglobus fulgidus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Z0T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1Z0T FirstGlance]. <br>
-
1Z0T is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Archaeoglobus_fulgidus Archaeoglobus fulgidus]. Active as [http://en.wikipedia.org/wiki/Endopeptidase_La Endopeptidase La], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.53 3.4.21.53] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Z0T OCA].
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3&#8491;</td></tr>
-
 
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1z0t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1z0t OCA], [https://pdbe.org/1z0t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1z0t RCSB], [https://www.ebi.ac.uk/pdbsum/1z0t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1z0t ProSAT]</span></td></tr>
-
==Reference==
+
</table>
-
Pathological crystallography: case studies of several unusual macromolecular crystals., Dauter Z, Botos I, LaRonde-LeBlanc N, Wlodawer A, Acta Crystallogr D Biol Crystallogr. 2005 Jul;61(Pt 7):967-75. Epub 2005, Jun 24. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15983420 15983420]
+
== Function ==
 +
[https://www.uniprot.org/uniprot/LONB_ARCFU LONB_ARCFU] ATP-dependent serine protease that mediates the selective degradation of mutant and abnormal proteins as well as certain short-lived regulatory proteins. Degrades polypeptides processively (By similarity).
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/z0/1z0t_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1z0t ConSurf].
 +
<div style="clear:both"></div>
 +
__TOC__
 +
</StructureSection>
[[Category: Archaeoglobus fulgidus]]
[[Category: Archaeoglobus fulgidus]]
-
[[Category: Endopeptidase La]]
+
[[Category: Large Structures]]
-
[[Category: Single protein]]
+
[[Category: Botos I]]
-
[[Category: Botos, I.]]
+
[[Category: Dauter Z]]
-
[[Category: Dauter, Z.]]
+
[[Category: LaRonde-LeBlanc N]]
-
[[Category: LaRonde-LeBlanc, N.]]
+
[[Category: Wlodawer A]]
-
[[Category: Wlodawer, A.]]
+
-
[[Category: atp-dependent protease]]
+
-
[[Category: b-type lon]]
+
-
[[Category: catalytic ser-lys dyad]]
+
-
 
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:11:02 2008''
+

Current revision

Crystal Structure of A. fulgidus Lon proteolytic domain

PDB ID 1z0t

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1z0t"
Personal tools