2h9x
From Proteopedia
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| - | [[Image:2h9x.png|left|200px]] | ||
| - | + | ==NMR structure for the CgNa toxin from the sea anemone Condylactis gigantea== | |
| + | <StructureSection load='2h9x' size='340' side='right'caption='[[2h9x]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[2h9x]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Condylactis_gigantea Condylactis gigantea]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2H9X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2H9X FirstGlance]. <br> | ||
| + | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=HYP:4-HYDROXYPROLINE'>HYP</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2h9x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2h9x OCA], [https://pdbe.org/2h9x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2h9x RCSB], [https://www.ebi.ac.uk/pdbsum/2h9x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2h9x ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [[https://www.uniprot.org/uniprot/TXNA_CONGI TXNA_CONGI]] Slows down the inactivation process of TTX-sensitive voltage-gated sodium channels (Nav). Has a strong crab-paralyzing activity.<ref>PMID:16814340</ref> | ||
| + | == Evolutionary Conservation == | ||
| + | [[Image:Consurf_key_small.gif|200px|right]] | ||
| + | Check<jmol> | ||
| + | <jmolCheckbox> | ||
| + | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/h9/2h9x_consurf.spt"</scriptWhenChecked> | ||
| + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | ||
| + | <text>to colour the structure by Evolutionary Conservation</text> | ||
| + | </jmolCheckbox> | ||
| + | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2h9x ConSurf]. | ||
| + | <div style="clear:both"></div> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | CgNa (Condylactis gigantea neurotoxin) is a 47-amino-acid- residue toxin from the giant Caribbean sea anemone Condylactis gigantea. The structure of CgNa, which was solved by 1H-NMR spectroscopy, is somewhat atypical and displays significant homology with both type I and II anemone toxins. CgNa also displays a considerable number of exceptions to the canonical structural elements that are thought to be essential for the activity of this group of toxins. Furthermore, unique residues in CgNa define a characteristic structure with strong negatively charged surface patches. These patches disrupt a surface-exposed cluster of hydrophobic residues present in all anemone-derived toxins described to date. A thorough characterization by patch-clamp analysis using rat DRG (dorsal root ganglion) neurons indicated that CgNa preferentially binds to TTX-S (tetrodotoxin-sensitive) voltage-gated sodium channels in the resting state. This association increased the inactivation time constant and the rate of recovery from inactivation, inducing a significant shift in the steady state of inactivation curve to the left. The specific structural features of CgNa may explain its weaker inhibitory capacity when compared with the other type I and II anemone toxins. | ||
| - | + | CgNa, a type I toxin from the giant Caribbean sea anemone Condylactis gigantea shows structural similarities to both type I and II toxins, as well as distinctive structural and functional properties(1).,Salceda E, Perez-Castells J, Lopez-Mendez B, Garateix A, Salazar H, Lopez O, Aneiros A, Standker L, Beress L, Forssmann WG, Soto E, Jimenez-Barbero J, Gimenez-Gallego G Biochem J. 2007 Aug 15;406(1):67-76. PMID:17506725<ref>PMID:17506725</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | <div class="pdbe-citations 2h9x" style="background-color:#fffaf0;"></div> | |
| - | + | == References == | |
| - | + | <references/> | |
| - | + | __TOC__ | |
| - | < | + | </StructureSection> |
[[Category: Condylactis gigantea]] | [[Category: Condylactis gigantea]] | ||
| - | [[Category: Gimenez-Gallego, G | + | [[Category: Large Structures]] |
| - | [[Category: Jimenez-Barbero, J | + | [[Category: Gimenez-Gallego, G]] |
| - | [[Category: Lopez-Mendez, B | + | [[Category: Jimenez-Barbero, J]] |
| - | [[Category: Perez-Castells, J | + | [[Category: Lopez-Mendez, B]] |
| + | [[Category: Perez-Castells, J]] | ||
[[Category: Beta sheet]] | [[Category: Beta sheet]] | ||
[[Category: Toxin]] | [[Category: Toxin]] | ||
Current revision
NMR structure for the CgNa toxin from the sea anemone Condylactis gigantea
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