2ar8

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[[Image:2ar8.png|left|200px]]
 
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{{STRUCTURE_2ar8| PDB=2ar8 | SCENE= }}
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==The structure of tryptophan 7-halogenase (PrnA)suggests a mechanism for regioselective chlorination==
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<StructureSection load='2ar8' size='340' side='right'caption='[[2ar8]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2ar8]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_fluorescens Pseudomonas fluorescens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AR8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2AR8 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=CTE:7-CHLOROTRYPTOPHAN'>CTE</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ar8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ar8 OCA], [https://pdbe.org/2ar8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ar8 RCSB], [https://www.ebi.ac.uk/pdbsum/2ar8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ar8 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/PRNA_PSEFL PRNA_PSEFL] Involved in the biosynthesis of the antifungal antibiotic pyrrolnitrin. Catalyze the chlorination of tryptophan (Trp) at C7 position to yield 7-chloro-L-tryptophan (7-CLT). The reaction between FADH2, Cl-, and O2 generates the powerful oxidant HOCl, which is presumed to carry out the chlorination reaction. The reaction of HOCl with the active site Lys-79 generates a lysine chloramine, which plays a key role in directing regiospecific chlorination of substrate in this important class of biosynthetic enzymes. It is also able to use bromide ions to generate monobrominated Trp.<ref>PMID:10941070</ref> <ref>PMID:9172332</ref> <ref>PMID:9537395</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ar/2ar8_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2ar8 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Chlorinated natural products include vancomycin and cryptophycin A. Their biosynthesis involves regioselective chlorination by flavin-dependent halogenases. We report the structural characterization of tryptophan 7-halogenase (PrnA), which regioselectively chlorinates tryptophan. Tryptophan and flavin adenine dinucleotide (FAD) are separated by a 10 angstrom-long tunnel and bound by distinct enzyme modules. The FAD module is conserved in halogenases and is related to flavin-dependent monooxygenases. On the basis of biochemical studies, crystal structures, and by analogy with monooxygenases, we predict that FADH2 reacts with O2 to make peroxyflavin, which is decomposed by Cl-. The resulting HOCl is guided through the tunnel to tryptophan, where it is activated to participate in electrophilic aromatic substitution.
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===The structure of tryptophan 7-halogenase (PrnA)suggests a mechanism for regioselective chlorination===
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Tryptophan 7-halogenase (PrnA) structure suggests a mechanism for regioselective chlorination.,Dong C, Flecks S, Unversucht S, Haupt C, van Pee KH, Naismith JH Science. 2005 Sep 30;309(5744):2216-9. PMID:16195462<ref>PMID:16195462</ref>
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{{ABSTRACT_PUBMED_16195462}}
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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==About this Structure==
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<div class="pdbe-citations 2ar8" style="background-color:#fffaf0;"></div>
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[[2ar8]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_fluorescens Pseudomonas fluorescens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AR8 OCA].
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== References ==
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<references/>
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==Reference==
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__TOC__
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<ref group="xtra">PMID:016195462</ref><ref group="xtra">PMID:015272170</ref><references group="xtra"/>
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Pseudomonas fluorescens]]
[[Category: Pseudomonas fluorescens]]
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[[Category: Dong, C.]]
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[[Category: Dong C]]
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[[Category: Flecks, S.]]
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[[Category: Flecks S]]
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[[Category: Haupt, C.]]
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[[Category: Haupt C]]
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[[Category: Naismith, J H.]]
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[[Category: Naismith JH]]
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[[Category: Pee, K H.Van.]]
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[[Category: Unversucht S]]
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[[Category: SSPF, Scottish Structural Proteomics Facility.]]
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[[Category: Van Pee KH]]
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[[Category: Unversucht, S.]]
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[[Category: Biosynthetic protein]]
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[[Category: Flavin-dependent halogenase]]
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[[Category: Helical bundle]]
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[[Category: Sandwiched sheet]]
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[[Category: Scottish structural proteomics facility]]
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[[Category: Sspf]]
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[[Category: Structural genomic]]
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[[Category: Tryptophan 7-halogenase]]
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Current revision

The structure of tryptophan 7-halogenase (PrnA)suggests a mechanism for regioselective chlorination

PDB ID 2ar8

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