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3zk0
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 3zk0 is ON HOLD until Paper Publication Authors: Blundell, K.L.I.M., Hough, M., Worrall, J.A.R. Description: The crystal structure of a Cu(I) metal...) |
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| - | '''Unreleased structure''' | ||
| - | The | + | ==The crystal structure of a Cu(I) metallochaperone from Streptomyces lividans in its apo form== |
| + | <StructureSection load='3zk0' size='340' side='right'caption='[[3zk0]], [[Resolution|resolution]] 1.70Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[3zk0]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_lividans Streptomyces lividans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZK0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3ZK0 FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7Å</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3zk0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zk0 OCA], [https://pdbe.org/3zk0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3zk0 RCSB], [https://www.ebi.ac.uk/pdbsum/3zk0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3zk0 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/Q93J41_STRCO Q93J41_STRCO] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | In Streptomyces lividans an extracytoplasmic copper-binding Sco protein plays a role in two unlinked processes: (i) initiating a morphological development switch and (ii) facilitating the co-factoring of the CuA domain of CcO (cytochrome c oxidase). How Sco obtains copper once secreted to the extracytoplasmic environment is unknown. In the present paper we report on a protein possessing an HX6MX21HXM motif that binds a single cuprous ion with subfemtomolar affinity. High-resolution X-ray structures of this extracytoplasmic copper chaperone-like protein (ECuC) in the apo- and Cu(I)-bound states reveal that the latter possesses a surface-accessible cuprous-ion-binding site located in a dish-shaped region of beta-sheet structure. A cuprous ion is transferred under a favourable thermodynamic gradient from ECuC to Sco with no back transfer occurring. The ionization properties of the cysteine residues in the Cys86xxxCys90 copper-binding motif of Sco, together with their positional locations identified from an X-ray structure of Sco, suggests a role for Cys86 in initiating an inter-complex ligand-exchange reaction with Cu(I)-ECuC. Generation of the genetic knockouts, Deltasco, Deltaecuc and Deltasco/ecuc, and subsequent in vivo assays lend support to the existence of a branched extracytoplasmic copper-trafficking pathway in S. lividans. One branch requires both Sco and to a certain extent ECuC to cofactor the CuA domain, whereas the other uses only Sco to deliver copper to a cuproenzyme to initiate morphological development. | ||
| - | + | Structural and mechanistic insights into an extracytoplasmic copper trafficking pathway in Streptomyces lividans.,Blundell KL, Hough MA, Vijgenboom E, Worrall JA Biochem J. 2014 May 1;459(3):525-38. doi: 10.1042/BJ20140017. PMID:24548299<ref>PMID:24548299</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| + | </div> | ||
| + | <div class="pdbe-citations 3zk0" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Streptomyces lividans]] | ||
| + | [[Category: Blundell KLIM]] | ||
| + | [[Category: Hough M]] | ||
| + | [[Category: Worrall JAR]] | ||
Current revision
The crystal structure of a Cu(I) metallochaperone from Streptomyces lividans in its apo form
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